高邮鸭主要免疫器官的表型发育及其与<em>TLRs</em>表达的相关性分析

doi:10.7668/hbnxb.2018.01.018

华北农学报 ›› 2018, Vol. 33 ›› Issue (1): 115-120. doi: 10.7668/hbnxb.2018.01.018

高邮鸭主要免疫器官的表型发育及其与TLRs表达的相关性分析

朱春红, 刘宏祥, 陶志云, 徐文娟, 宋卫涛, 章双杰, 李慧芳

江苏省家禽科学研究所, 江苏扬州 225125

收稿日期:2017-12-11 出版日期:2018-02-28
通讯作者: 李慧芳(1974-),女,山西阳泉人,研究员,博士,主要从事家禽遗传育种与家禽资源保护研究。
作者简介:朱春红(1982-),女,江苏盐城人,副研究员,博士,主要从事家禽分子抗病育种研究。
基金资助:
扬州市科技支撑项目（YZ2015039）

Development of Immune Tissue and Correlation Analysis of TLRs Expression in Gaoyou Duck

ZHU Chunhong, LIU Hongxiang, TAO Zhiyun, XU Wenjuan, SONG Weitao, ZHANG Shuangjie, LI Huifang

Jiangsu Institute of Poultry Sciences, Yangzhou 225125, China

Received:2017-12-11 Published:2018-02-28

摘要/Abstract

摘要： Toll样受体（TLRs）在机体天然免疫中发挥重要作用。为分析TLRs在鸭主要免疫器官中的表达情况，在测定和分析高邮鸭免疫器官发育的基础上，荧光定量PCR法检测了高邮鸭TLR1、TLR2、TLR4、TLR5（TLR1/2/4/5）4种TLR mRNA基因在免疫器官（胸腺、脾脏和法氏囊）中的相对表达情况，并进行相关性分析。结果表明，TLR1/2/4/5 mRNA在不同发育时间段都有表达，但不同TLR基因在不同免疫组织中表达模式存在差异，总体来讲，胸腺中TLR1/2/4/5 mRNA在1周龄时表达水平较高，脾脏中TLR1/2/5 mRNA在8周龄时表达量显著高于其他周龄，法氏囊中TLR1/5在8周龄时表达量较高。相关性分析结果展示：免疫器官绝对增重和体重发育呈现高相关系数；TLR1/2/4/5 mRNA的表达水平和体重发育相关系数较高，大多高于TLRs mRNA表达水平与免疫器官增重的相关系数；胸腺组织中TLR1/2/4/5 mRNA表达量两两相关系数值相对较高。研究结果可以揭示TLRs在机体及其免疫器官发育过程中的表达情况，为进一步分析和揭示其在免疫抗感染过程中的作用奠定基础。

关键词: 高邮鸭, 免疫器官, 发育进程, TLRs mRNA相对表达

Abstract: TLRs play an important role in natural immunity.Development of body, thymus, spleen, bursa fabriciu were measured, and the expression levels of TLR1, TLR2, TLR4, TLR5 (TLR1/2/4/5)were detected in these immune tissues by fluorogenic qRT-PCR in Gaoyou duck. The results revealed that the expressions of TLR1/2/4/5 mRNA were detected and showed different pattern in thymus, spleen, bursa fabricius during production period (8 weeks). Expression of TLR1/2/4/5 in thymus at 1 week was higher than those in other weeks, and the higher expressions of TLR1/2/5 mRNA in spleen were showed at 8 weeks, expression of TLR1/5 in bursa fabricius was higher at 8 weeks. Analysis of correlation revealed that there were high index between body weight and immune tissue weight, while the index of expression of TLR1/2/4/5 mRNA and weights of immune tissue were less than the index of expression of TLR1/2/4/5 mRNA and body weight. The expression of TLR1/2/4/5 mRNA in thymus exhibited relatively high correlated index with each other. In conclude, these results can reveal expression pattern of TLR1/2/4/5 during the process of immune organ development.

Key words: Gaoyou duck, Immune tissue, Development, Relative expression of TLRs mRNA

中图分类号:

S834.03

朱春红, 刘宏祥, 陶志云, 徐文娟, 宋卫涛, 章双杰, 李慧芳. 高邮鸭主要免疫器官的表型发育及其与TLRs表达的相关性分析[J]. 华北农学报, 2018, 33(1): 115-120. doi: 10.7668/hbnxb.2018.01.018.

ZHU Chunhong, LIU Hongxiang, TAO Zhiyun, XU Wenjuan, SONG Weitao, ZHANG Shuangjie, LI Huifang. Development of Immune Tissue and Correlation Analysis of TLRs Expression in Gaoyou Duck[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 115-120. doi: 10.7668/hbnxb.2018.01.018.

http://www.hbnxb.net/CN/Y2018/V33/I1/115

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