摘要： HKT基因家族在植物维持体内Na⁺/K⁺平衡的过程中发挥着重要作用。为了研究普通小麦HKT基因家族在小麦耐盐机制中的作用，采用转化酵母突变菌株G19的方法，对普通小麦HKT基因家族的不同成员，同一成员在不同染色体上的拷贝以及同一成员等位基因的Na⁺亲和力进行了比较分析。普通小麦HKT基因家族的3个成员（HKT1；5、HKT2；1和HKT2；2）与Na⁺亲和力不同。HKT2；2-A的Na⁺亲和力高于HKT2；2-B。单氨基酸突变Leu⁴⁸→Pro⁴⁸提高了HKT2；1对Na⁺的亲和力，而突变His²¹⁵→Tyr²¹⁵降低了HKT2；1对Na⁺的亲和力，这表明，Leu⁴⁸和His²¹⁵可能与普通小麦HKT2；1 Na⁺转运功能有关

关键词: 小麦, HKT, Na⁺亲和力, 酵母Na⁺耐受性

Abstract: The members of HKT family play an important role in maintaining Na⁺/K⁺ homeostasis.In order to analyze the role of wheat HKT genes in salt-tolerance mechanism,comparative analysis on Na⁺ affinity of wheat different members,the copies on different genomes and the member's alleles of wheat HKT family were conducted by transforming yeast mutant G19.Three members of HKT family, HKT1; 5, HKT2; 1,and HKT2; 2,showed different Na⁺affinity.Na⁺affinity of HKT2;2-A was higher than HKT2;2-B.Leu⁴⁸→Pro⁴⁸ mutation increased Na⁺ affinity while His²¹⁵→Tyr²¹⁵ mutation decreased that of HKT2;1.The results indicated that the two amino acids may be related to Na⁺ transporter in common wheat.

Key words: Wheat, HKT, Na⁺affinity, Yeast Na⁺ tolerance

中图分类号: 

• Q78

• S512.03