甜瓜PI414723抗霜霉病基因SSR分子标记

doi:10.7668/hbnxb.2016.S1.015

摘要/Abstract

摘要： 为了研究野生甜瓜PI414723抗性遗传规律，找到与抗病基因连锁的分子标记，并对抗病基因进行定位。以野生甜瓜PI414723和新疆哈密瓜农家品种卡拉克赛为材料构建BCr、BCs和F₂，苗期接种甜瓜霜霉病进行抗性鉴定、统计分析，基于ICuGI已构建遗传连锁图谱，应用集团分离法和1 090对甜瓜SSR引物进行连锁遗传分析。结果表明：PI414723对霜霉病的抗性由隐性单基因控制，引物DM0231扩增出的多态性条带与抗病基因表现连锁关系，该多态性片段大小为226 bp，遗传连锁距离为2.67 cM，并将抗病基因定位在LG9上。DM0231可作为甜瓜抗霜霉病分子育种的分子标记，也为进一步对抗霜霉病基因精细定位奠定了基础。

关键词: 甜瓜, 霜霉病, 抗病基因, SSR分子标记

Abstract: In order to study the inheritance of wild melon PI414723,founding the molecular markers linked to resistance genes and locating the resistance genes.The F₂,BCr and BCs populations were derived by crossing and back crossing with Cucumis melo ssp. melo lines PI414723 and kalakesai.Investigation and statistical analyses scored for response to inoculation with[Pseudoperonospora cubensis (Berk.etCurt.) Rostov.].Based on the genetic linkage map that was constructed by the ICuGI.SSR analysis,combined with bulked segregation analysis(BSA),was done on the DNA of F₂ using 1 090 pairs of SSR primers.The results indicated that the resistance to downy mildew in PI414723 was controlled by one single recessive gene. An unique 226 bp fragment was amplified with the primer DM0231.It was identified that was linked to resistance gene of PI414723 at a distance of 2.67 cM.The resistance gene was located to LG9.This SSR marker DM0231could be useful for marker assisted selection in melon breeding for downy mildew resistance.

Key words: Cucumis melo ssp. melo, Downy mildew, Disease resistance gene, SSR marker

中图分类号:

Q78
S652.03

张学军, 杨俊涛, 李寐华, 杨永, 伊鸿平. 甜瓜PI414723抗霜霉病基因SSR分子标记[J]. 华北农学报, 2016, 31(S1): 86-91. doi: 10.7668/hbnxb.2016.S1.015.

ZHANG Xuejun, YANG Juntao, LI Meihua, YANG yong, YI Hongping. SSR Markers Linked of the Resistance Gene for Downy Mildew in Cucumis melo ssp.melo PI414723[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 86-91. doi: 10.7668/hbnxb.2016.S1.015.

http://www.hbnxb.net/CN/Y2016/V31/IS1/86

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