花期前后稻穗水分含量与水通道蛋白基因家族表达关系研究

doi:10.7668/hbnxb.2016.02.008

摘要/Abstract

摘要： 水稻开花期对水分胁迫非常敏感直接影响到结实率,水分供应主要通过水通道蛋白来完成,为了研究花期稻穗的相对含水量与水通道蛋白基因家族表达关系,探讨了花期前后6 d内稻穗相对含水量和水通道蛋白基因家族表达变化情况。结果表明:稻穗相对含水量呈现出花前最高、开花期当天最低、花后则再出现增加的规律;OsPIPs基因家族中各个基因表达的水平各有不同,其中OsPIP1-1、OsPIP1-2、OsPIP2-1、OsPIP2-4和OsPIP2-7的表达水平较高,而OsPIP2-2、OsPIP2-3和OsPIP2-8这3个基因在花期前后6 d内均没有表达;所有OsTIPs基因均表达,但不同OsTIPs基因表达水平在不同时间点也不同,除了OsTIP1-2和OsTIP3-2的表达量比较低外,其他基因的表达量都非常高;OsNIPs基因家族表达量相对较低,如OsNIP1-2、OsNIP1-4、OsNIP3-2及OsNIP3-3在花期前后均不表达,而OsNIP1-1和OsNIP2-1在开花前后均有表达,并在开花当天表达量较高;OsNIP2-2和OsNIP3-1只在开花当天表达,其他时间点均不表达;OsSIP1-1、OsSIP1-2在花前3 d表达量最大,而OsSIP2-1在开花当天表达量最大,其他时间点略有下降。结果说明,花期前后稻穗内OsTIPs基因家族表达量最大,然后是OsPIPs基因家族,再次是OsSIPs和OsNIPs基因家族,但不同水通道蛋白家族各基因间的表达模式也不同,可能与对水分运输分工差异有关,其中液泡膜水通道蛋白在水稻花期水分运输中的作用可能最大。

关键词: 水稻, 稻穗, 水通道蛋白, 相对含水量, 基因表达

Abstract: Rice flowering period is very sensitive to water stress and directly effect on setting rate;water supplies are affected by aquaporin genes expression and its activities.In order to investigate relationship between the relative water content and the aquaporin genes expression in rice panicle, the genes expression level of aquaporin and the relative water content in rice panicle were studied during the time before and after flowering for 6 days.The results showed that:The relative water content in pancile of rice was highest before flowering, and then lowest in the day of flowering, but after flowering the relative water content increased to highest value;Different OsPIPs showed at different expressional patterns, for example, OsPIP1-1, OsPIP1-2, OsPIP2-1, OsPIP2-4 and OsPIP2-7 displayed high level during these time.However, OsPIP2-2, OsPIP2-3 and OsPIP2-8 did not show any expression throughout the flowering period;All OsTIPs were expressed throughout the flowering period, but there were some differences in the expression level in different time, compared to high expression of others OsTIPs, OsTIP1-2 and OsTIP3-2 kept very lower expression level during these time;The expression of OsNIPs displayed very lower level during these time, for example OsNP1-2, OsNIP1-4, OsNIP3-2 and OsNIP3-3 were not expressed throughout the flowering period, OsNIP1-1 and OsNIP2-1 could express in the whole time, However, OsNIP2-2 and OsNIP3-1 could have a certain amount of expression only in flowering day, rather than in the other times;OsSIP1-1, OsSIP1-2 showed at the high value before the flowering day, while OsSIP2-1 reached its maximum during the flowering day.This experiment results showed that, the highest expression level was OsTIPs, followed by OsPIPs, then were OsSIPs and OsNIPs, but different water channel protein genes showed at different expression pattern during these time, and OsTIPs that displayed high expression level might be need more tonoplast intrinsic protein activities to keep water supply during the flower time of rice panicle.

Key words: Rice, Rice panicle, Aquaporin, Relative water content, Gene expression

中图分类号:

Q78
S511.03

赵亚波, 李培纲, 陈泽恺, 潘伟槐, 莫亿伟. 花期前后稻穗水分含量与水通道蛋白基因家族表达关系研究[J]. 华北农学报, 2016, 31(2): 38-44. doi: 10.7668/hbnxb.2016.02.008.

ZHAO Yabo, LI Peigang, CHEN Zekai, PAN Weihuai, MO Yiwei. The Variation of Relative Water Content and Expression of Aquaporins Genes in Rice Panicle During before and after Flowering[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 38-44. doi: 10.7668/hbnxb.2016.02.008.

http://www.hbnxb.net/CN/Y2016/V31/I2/38

参考文献

[1] Ahmadi N,Audebert A,Bennett M J,et al.The roots of future rice harvests[J].Rice,2014,7(29):1-9.
[2] 张卫星,朱德峰,徐一成,等.不同水分条件下水稻籽粒形态及其与粒重的关系[J].作物学报,2008,34(10):1826-1835.
[3] 朱美君,康蕴,陈珈,等.植物水通道蛋白及其活性调节[J].植物学通报,1999,16(1):44.
[4] 尤真真,高蓉,田珊,等.植物水通道蛋白结构与功能及其识别与转导水稻黄单胞菌Hpa1信号的机制[J].植物病理学报,2013,43(3):232-248.
[5] 李军,乙引.植物水通道蛋白的运输和调节[J].植物生理学通讯,2010(5):493-498.
[6] 杨淑慎,山仑,郭蔼光,等.水通道蛋白与植物的抗旱性[J].干旱地区农业研究,2005,23(6):214-218.
[7] 吴安慧,张岁岐,邓西平,等.水分亏缺条件下玉米根系PIP2-5基因的表达(简报)[J].植物生理学通讯,2006,42(3):457-460.
[8] 李仁,吴新新,李蔚,等.番茄水通道蛋白基因SlAQP的克隆与序列分析[J].中国农业科学,2012,45(2):302-310.
[9] 李嵘,牛向丽,苗雁文,等.水通道蛋白基因OsPIP2; 6的功能分析[J].中国农业科学,2013,46(15):3079-3086.
[10] Liu C,Fukkumoto T,Matumoto T,et al.Aquaporin OsPIP1;1 promotes rice salt resistance and seed germination[J].Plant Physiology and Biochemistry,2013,63(4):151-158.
[11] 周建霞,张玉屏,朱德峰,等.高温下水稻开花习性对受精率的影响[J].中国水稻科学,2014,28(3):297-303.
[12] Mitra J.Genetics and genetic improvement of drought resistance in crop plants[J].Current Science,2001,80:758-763.
[13] Mo Y W,Liang G B,Shi W Q,et al.Metabolic responses of alfalfa(Medicago Sativa L.)leaves to low and high temperature induced stresses[J].African Journal of Biotechnology,2011,10(7):1117-1124.
[14] 王贺正,马均,李旭毅,等.水分胁迫对水稻籽粒灌浆及淀粉合成有关酶活性的影响[J].中国农业科学,2009,42(5):1550-1558.
[15] Sakurai J,Ishikawa F,Yamaguchi T,et al.Identification of 33 rice aquaporin genes and analysis of their expression and function[J].Plant & Cell Physiology,2005,46(9):1568-1577.
[16] Jarzyniak K M,Jasinsk M.Membrane transporters and drought resistance-a complex issue[J].Frontiers in Plant scicence,2014,5(12):1-15.
[17] 陈义芳,顾蕴洁,王忠.四个不同粒重水稻品种颖果发育的比较[J].中国水稻科学,2009,23(4):405-413.
[18] 冯九焕,卢永根,刘向东,等.水稻花粉发育过程及其分期[J].中国水稻科学,2001,15(1):21-28.
[19] 冯芳玖,李静婷,郭丹凤,等.年份气温差异对野生型和转OsPIN1a基因水稻胚乳发育及种子萌发的影响[J].核农学报,2015,29(11):2198-2207.
[20] 张彬,芮雯奕,郑建初,等.水稻开花期花粉活力和结实率对高温的响应特征[J].作物学报,2007,33(7):1177-1181.
[21] 李贤勇,李顺武,何永歆,等.穗层自然高温对杂交水稻结实的影响[J].西南农业学报,2008,21(1):44-47.
[22] Cui X,Hao F,Chen H,et al.Expression of the Vicia faba VfPIP1 gene in Arabidopsis thaliana plants improves their drought resistance[J].Journal of Plant Research,2008,12(12):207-214.
[23] Heinen R B,Bienertg P,Cohen D,et al.Expression and characterization of plasma embrane aqua-porins in stomatal complexes of Zea mays[J].Plant Molecular Biology,2014,86(3):335-350.
[24] Gao Z,He X,Zhao B,et al.Over expressing a putative aquaporin gene from wheat,TaNIP,enhances salt tolerance in transgenicArabidopsis[J].Plant & Cell Physiology,2010,51(5):767-775.
[25] Liu H Y,Yu X,Cui D Y,et al.The role of water channel proteins and nitric oxide signaling in rice seed germination[J].Cell Research,2007,17(7):638-649.
[26] Lin W,Peng Y,Lig,et al.Isolation and functional characterization of PgTIP1,ahormone-autotrophic cells-specific tonoplast aquaporins in ginseng[J].Journal of Experimental Botany,2007,58(5):947-956.
[27] Mahdieh M,Mostajeran A,Horie T,et al.Drought stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum plants[J].Plant and Cell Physiology,2008,49(5):801-813.
[28] Lian H L,Yu X,Lane D,et al.Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment[J].Cell Research,2006,16(7):651-660.
[29] Xu W,Dai W,Yan H,et al.Arabidopsis NIP3;1 plays an important role in Arsenic uptake and Root-to-shoot translocation under arsenite stress conditions[J].Molecular Plant,2015,8(5):722-733.
[30] Chaumont F,Barrieu F,Wojcik E,et al.Aquaporins constitute a large and highly divergent protein family in maize[J].Plant Physiology,2001,125(3):1206-1215.
[31] Peng Y H,Lin W L,Cai W M,et al.Overexpression of a panax ginseng tonoplast aquaporin alters salt tolerance,drought tolerance and cold acclimation ability in transgenic Arabidopsis plants[J].Planta,2007,226(3):729-740.
[32] Ma S,Quist T M,Ulanov A,et al.Loss of TIP1;1 aquaporin in Arabidopsis leads to cell and plant death[J].Plant Journal,2004,40(6):845-859.

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