摘要： 为给甘肃高山细毛羊生长标记候选基因的筛选提供参考依据,研究了绵羊GH基因第3内含子突变对甘肃高山细毛羊生长性状可能产生的影响。采用PCR-SSCP技术检测甘肃高山细毛羊及其与特克赛尔、邦德和澳洲美利奴羊的杂交品种GH基因第3内含子多态性,分析GH基因第3内含子突变对羔羊初生重、3月龄重、断奶重及日增重的影响。结果表明,甘肃高山细毛羊及其杂种羔羊GH基因第3内含子第98 bp处有一个C>T突变,共有AA、AB和BB 3种基因型,甘肃高山细毛羊和对照组杂种羊的GH基因第3内含子PIC值均为0.25~0.50,为中度多态。其中BB型为优势基因型。等位基因存在与缺失对体重及日增重的关联分析表明,存在等位基因B的甘肃高山细毛羊的3月龄重、断奶重及日增重均显著或极显著高于缺失等位基因B的个体(P<0.05或P<0.01),缺失等位基因A的特甘细绵羊在初生重、断奶重及日增重方面显著高于存在等位基因A的个体(P<0.05)。邦甘细绵羊中,存在等位基因B的个体3月龄重、断奶重、日增重均显著高于等位基因B缺失个体。相关性分析表明,甘肃高山细毛羊不同基因型与初生重间关联度不显著,BB型甘肃高山细毛羊3月龄重和断奶重均显著高于AA型(P<0.05),AA型甘肃高山细毛羊日增重与BB型也显著相关(P<0.05)。对照组中,特甘细羊BB型的初生重、断奶重及日增重均显著或极显著高于AA型特甘细(P<0.05或P<0.01)。邦甘细、澳甘细不同基因型与体重及日增重均无相关性(P>0.05)。结果显示GH基因第3内含子可作为甘肃高山细毛羊生长标记候选基因。

关键词: 甘肃高山细毛羊, GH基因第3内含子, 多态性, 生长性状

Abstract: Intron 3 of the ovine GH gene mutation of Gansu alpine fine wool sheep growth traits may have effects and the gene markers to provide reference of Gansu alpine fine wool sheep growth candidate.Gansu alpine merino and Texel merino,Bond merino,Australia merino crossbred sheep GH gene intron 3 polymorphism was detected by PCR-SSCP technique,analysis of GH gene mutation in intron 3 of lamb birth weight,three-month weight,weaning weight and daily gain influence.The results showed that the first mutation between the Gansu alpine merino and crossbred lamb was GH gene intron 3 of 98 bp a C>T mutation,and this mutation gives AA,AB and BB three genotypes,Gansu alpine merino and crossbred sheep in the control group that the PIC values were between 0.25-0.50,was moderate polymorphism.The dominant genotype of which was BB.Alleles associated with the presence or absence of body weight and daily gain analysis showed the presence of allele B of Gansu alpine merino 3-month weight,weaning weight and daily gain were significantly higher than the individual's lack of allele B(P<0.05),deletion allele a special Texel Gansu alpine merino in birth weight,weaning weight and average daily gain was significantly higher than that of the existence of areas a allele individuals(P<0.05).Bond Gansu alpine merino of allele B 3-month weight,weaning weight,average daily gain were significantly higher than the B allele missing individuals.Correlation analysis showed that different genotypes of Gansu alpine merino correlation between birth weight was not significant,BB type Gansu alpine merino of 3-month weight and weaning weight were significantly higher than AA genotype(P<0.05),average daily gain of AA and BB genotypes Gansu alpine merino were also significantly correlated(P<0.05).In the control group,Bond Gansu alpine merino BB type of birth weight,weaning weight and average daily gain were significantly higher than those of AA genotype(P<0.01).Bond Gansu alpine merino,Australian merino,Gansu alpine merino different genotypes with body weight and average daily gain were no correlation(P>0.05).The results showed that the intron 3 of GH gene could be used as a marker of growth traits of Gansu Alpine Merino candidate gene.

Key words: Gansu alpine merino, GH gene intron 3, Polymorphism, Growth traits

中图分类号: 

• Q78

• S826