摘要： 为了提高甘薯的抗逆能力,通过RACE的方法克隆到一个含有AP2结构域的ERF家族基因,该家族基因在植物逆境调控中起重要作用。该基因cDNA全长1 025 bp,编码区为669 bp,编码223个氨基酸,该基因在甘薯中未曾报道,将其命名为 IbERF3。通过进化树分析发现, IbERF3 在茄目类作物中具有一定的保守性。通过定量PCR研究发现, IbERF3 在甘薯根、叶中都有表达,且在叶片中的表达量最高,同时研究发现,在干旱、盐胁迫处理后 IbERF3 在根和叶片中表达量都显著上升。在用植物激素ABA处理后, IbERF3 的表达量逐渐上升并在24 h时达到最大。因此,推测 IbERF3 在甘薯抗逆途径中起重要作用。

关键词: 甘薯, ERF, 基因克隆, 表达, 非生物逆境

Abstract: In order to improve the stress resistance of sweet potato,we used RACE method to clone IbERF3 contained an AP2 domain which belong to ERF superfamily which plays an important role in stress resistance.Blast showed that IbERF3 was a new gene in sweet potato that had never been reported.The cDNA length of IbERF3 was 1 205 bp,and contained an 669 bp open reading frame encoding a 223 amino acids.By homology analysis showed that IbERF3 was conserved in Solanaceae plants.Constitutive expression showed that IbERF3 express in root,leaf and stem,and the expression of IbERF3 was the highest in leaf.The result reveal that the expression of IbERF3 was induced by drought,salt both in leaf and root by qRT-PCR.After treating with ABA,The expression of IbERF3 increased gradually and reached a maximum at 24 h.It suggests that IbERF3 plays significant roles in responses to abiotic stress in sweet potato.

Key words: Sweet potato, ERF, Gene cloning, Expression, Abiotic stress

中图分类号: 

• Q78

• S531.03