菌剂与肥料配施对露天矿排土场苜蓿生长及土壤养分含量的影响

doi:10.7668/hbnxb.2014.04.033

摘要/Abstract

摘要： 为解决露天煤矿排土场复垦土壤植被恢复困难的问题，探索有效地改良土壤的措施，采用盆栽试验方法测定排土场土壤中菌剂与肥料配施对紫花苜蓿的生长发育的影响，进而探讨菌根剂与肥料的配施对矿区土壤的改良作用。结果表明：菌根剂与肥料配合施用能显著提高紫花苜蓿的株高、生物量、叶绿素、蛋白质、可溶性糖的含量以及抗氧化酶同工酶活性等，土壤中速效磷、速效氮、有机质、全氮、全磷含量以及土壤微生物数量也都有明显提高。其中尤以有机肥+无机肥+菌剂配施效果最为显著，与对照相比，株高、鲜质量、干质量分别增加47%，277%，280%，叶绿素、蛋白质、可溶性糖含量分别增加36%，183%，59%，过氧化氢酶、过氧化物酶、超氧化物歧化酶活性分别增加281%，1250%，15%，土壤速效N、速效P含量分别增加130%，217%，有机质含量增加76%，全N、全P含量分别增加102%，160%，土壤细菌、放线菌、真菌数量分别增加103%，68%，20%。总之，有机肥+无机肥+菌剂配施显著促进了紫花苜蓿的生长，提高了排土场土壤的养分含量，是矿区复垦土壤植被恢复中比较好的组合方式。

关键词: 丛枝菌根真菌, 有机肥, 无机肥, 排土场土壤, 紫花苜蓿

Abstract: Opencast coal mining leaves large areas of refuse dump,because of the environment pollution,soil erosion、nutrient-poor of soil and biodiversity loss,vegetation reclamation in these areas is difficult,so explore the measures for soil improvement is important to practice.In this paper,the effect of combined application of microbial inoculum and fertilizer on growth of alfalfa in refuse dump through pot culture experiment,and then explore the effect of combined application of microbial inoculum and fertilizer on improvement of mine soil.The results showed that combined application of microbial inoculum and fertilizer significantly increased the alfalfa's biomass,plant height,the content of chlorophyll,protein,soluble sugar and activity of antioxidant enzyme isozyme,nutrient content of reclaimed soil was also significantly increased.The most effective treatments was organic fertilizer+inorganic fertilizer+microbial inoculum applied,compared with the control,the increasing rates of plant height,fresh weight and dry weight reached 47%,277% and 280%,the increasing rates of chlorophyll content,protein content and soluble sugar content reached 36%,183% and 59%,the increased rates of hydrogen peroxide activity,peroxidase activity and superoxide dismutase activity reached 281%,1250% and 15%,the increased rates of soil available N,P,organic matter,total N and P reached 130%,217%,76%,102% and 160%,the increased rates of bacteria,actinomycetes and fungi reached 103%,68% and 20%.In general,combined application of microbial inoculum,inorganic fertilizer and organic fertilizer significantly promoted the growth of alfalfa and improved the fertility of dump soil,was a good combination of vegetation reclamation in mine wasteland soil.

Key words: Arbuscular mycorrhizal fungi, Organic fertilizer, Inorganic fertilizer, Reclamation mine soil, Alfalfa

中图分类号:

S551

王海娟, 包玉英, 牛天心, 李秀芳. 菌剂与肥料配施对露天矿排土场苜蓿生长及土壤养分含量的影响[J]. 华北农学报, 2014, 29(4): 186-191. doi: 10.7668/hbnxb.2014.04.033.

WANG Hai-juan, BAO Yu-ying, NIU Tian-xin, LI Xiu-fang. Effect of Combined Application of Microbial Inoculum and Fertilizer on Fertility of Open-pit Mine Dump Reclaimed Soil[J]. journal1, 2014, 29(4): 186-191. doi: 10.7668/hbnxb.2014.04.033.

http://www.hbnxb.net/CN/Y2014/V29/I4/186

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