华北农学报 ›› 2011, Vol. 26 ›› Issue (S1): 29-34. doi: 10.7668/hbnxb.2011.S1.006

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松浦鲤保种群体遗传结构研究

贾智英1, 李飞1,2, 李池陶1, 石连玉1   

  1. 1. 中国水产科学研究院黑龙江水产研究所, 黑龙江 哈尔滨 150070;
    2. 上海海洋大学水产与生命学院, 上海 201306
  • 收稿日期:2011-06-03 出版日期:2011-12-30
  • 通讯作者: 石连玉(1961-),男,辽宁人,研究员,主要从事鱼类遗传育种研究。
  • 作者简介:贾智英(1976-),女,山东烟台人,副研究员,博士,主要从事鱼类遗传育种与生物技术研究。
  • 基金资助:
    现代农业产业技术体系建设专项资金资助(CARS-46-02);黑龙江省自然科学基金项目(C201044)

Study on the Genetic Diversity of the Conservation Population in Songpu Common Carp

JIA Zhi-ying1, LI Fei1,2, LI Chi-tao1, SHI Lian-yu1   

  1. 1. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
    2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
  • Received:2011-06-03 Published:2011-12-30

摘要: 松浦鲤作为鲤鱼的一个培育品种,在我国北方地区的鱼类养殖实践中占有重要地位,为了促进该品种优良性状的稳定保持和种质资源的可持续利用,本研究应用29对多态性较高的微卫星标记对松浦鲤保种群体进行了遗传结构研究。结果显示:在194尾松浦鲤个体中,共检测到166个等位基因,平均有效等位基因数为5.724 1,平均多态信息含量为0.584 9,平均期望杂合度为0.572 2;哈迪-温伯格平衡检验结果群体处于不平衡状态;瓶颈效应分析表明,群体已经历了瓶颈效应;根据连锁不平衡方法计算有效群体大小为69.8。该研究表明人工选择压力对松浦鲤保种群体造成了较大影响,虽然其仍具有较丰富的遗传多样性,但由于经历了瓶颈效应,这对于品种的长期发展极为不利,因此在该品种保种工作中应注重加强保种策略研究,从而有利于进一步保持其丰富的遗传多样性和保持其种质利用价值。

关键词: 松浦鲤, 保种, 微卫星, 遗传结构

Abstract: Songpu common carp is a breeding variety.In order to maintain the genetic diversity of the variety for a long time,the genetic structure of the fish was studies with 29 polymorphic microsattelite loci.The study showed that in the 194 sampled individuals,166 alleles were detected,the average number of effective allele is 5.724 1,average PIC is 0.584 9,and average expected heterozygosity is 0.572 2,which indicated that the new variety has a relative high genetic diversity.Hardy-weinberg equilibrium analysis showed that the population was in disequilibrium mode,which indicated that artificial selection had a great effect on the population.Bottleneck analysis illustrated that the population has experienced bottleneck effect recently.The effective population size was 69.8 with linkage disequilibrium method.The study illustrated that the variety has rich genetic diversity,but the population structure was disequilibrium and bottleneck effect has happened.Therefore,it′s necessary to take measures for reservation of the genetic diversity of the new variety.

Key words: Songpu common carp, Reservation, Microsatellite, Genetic structure

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引用本文

贾智英, 李飞, 李池陶, 石连玉. 松浦鲤保种群体遗传结构研究[J]. 华北农学报, 2011, 26(S1): 29-34. doi: 10.7668/hbnxb.2011.S1.006.

JIA Zhi-ying, LI Fei, LI Chi-tao, SHI Lian-yu. Study on the Genetic Diversity of the Conservation Population in Songpu Common Carp[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 29-34. doi: 10.7668/hbnxb.2011.S1.006.

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