哲罗鱼生长速度相关性状的微卫星标记筛选

doi:10.3969/j.issn.1000-7091.2012.z1.007

摘要/Abstract

摘要： 利用22个微卫星标记对随机交配的野生哲罗鱼F1176个个体的生长速度相关性状(体质量、全长、体高、体宽、头长、头宽和眼间距)进行分析，22个微卫星标记共检测到67个等位基因，各座位的等位基因2～6个，片段大小为109～452 bp，平均观测杂合度和期望杂合度分别为0.583 5和0.564 0，22个位点均有不同程度的偏离Hardy-Weiberg平衡。利用SPSS软件对7个性状正态分布检测发现，7个性状都显示出连续变异的特点，属典型的数量性状或者多基因遗传，符合正态分布；同时用GLM程序对22个微卫星标记与生长速度相关性状进行相关性分析，并将与性状显著相关的标记进行基因型之间的多重比较，结果显示，22个微卫星座位中，有11个标记与至少一种性状显著相关，其中与全长相关的标记最少为4个，与体高和体宽相关的标记最多的为7个；多重比较可知每个标记中同一性状均有部分基因型差异达到显著水平，不同标记所代表均值也有些差异。上述这些标记为进一步的分子标记辅助育种提供了基础。

关键词: 哲罗鱼, 微卫星标记, 性状, 标记辅助育种

Abstract: 22 microsatellite markers were used to analyze the growth rate related traits of 176 individuals fromF1 of random mating Hucho taimen， including body weight， body length， body height， body width， head length， headwidth and interorbital width． The results showed that of the 67 alleles detected in total， the numbers of alleles rangedfrom 2 to 6 per locus with the fragments of 109 to 452 bp， and the mean observed heteozygosity and the mean expected heteozygosity were 0． 583 5 and 0． 564 0， respectively． Deviations from Hardy-Weinberg equilibrium ( HWE)occurred at 22 loci in different degrees． The normal distribution of 7 traits was analyzed using SPSS software and theresults indicated that all the seven traits had a characteristic of continuous variation belonging to the typical quantitative traits or polygenic inheritance traits， and conformed to the normal distribution． Meanwhile， GLM linear modelwas constructed between the 22 microsatellite markers and the growth rate related traits， and the markers significantly correlative to the traits were performed multiple comparison based on the genotypes． The results revealed that of22 microsatellite loci， there were 11 markers significantly correlative to one trait at least， and the minimum numberof markers related to body length was 4 and the maximum number of markers related to body height and body widthwas 7． Through the multiple comparisons， it was found that there was significant difference of genotypes from thesame trait in every locus， and there was also difference among the mean value of the different markers． The screeningof these markers could provide some basis for the further study on molecular marker-assisted breeding．

Key words: Hucho taimen, Microsatellite markes, Traits, Marker assisted selection

中图分类号:

S917.4

TONG Guang-xiang, KUANG You-yi, ZHANG Chao, YIN Jia-sheng. Identification of Microsatellite Markers Associated with Growth Rate Traits in Hucho taimen[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.007.

http://www.hbnxb.net/CN/Y2012/V27/IS1/33

参考文献

[1] O ’ Connell M, Wright J M．Microsatellite DNA in fishes [J]．Rev Fish Biol Fish, 1997(7) : 331 － 363．
[2] 孙效文, 梁利群．鲤鱼的遗传连锁图谱( 初报) [J]．中国水产科学, 2000, 7(1 ) : 1 － 5．
[3] Naish K A, Skibinski O．Tetranucleiotide microsatellitesloci for Indian major carp ( Catlacatla) [J]．J Fish Biol，1998(53) : 886 － 889．
[4] Agresti J J, Seki S, Cnaani A, et al．Breeding new strainsof tilapia: development of an artificial center of origin andlinkage map based on AFLP and microsatellite loci[J]．Aquaculture, 2000(185) : 43 － 56．
[5] 许可, 马爱军, 王新安, 等．大菱鲆生长性状相关的微卫星标记筛选[J]．海洋与湖沼, 2009, 40(5 ) : 577 －583．
[6] 樊佳佳, 白俊杰, 李小慧, 等．大口黑鲈生长性状的微卫星 DNA 标记筛选[J]．遗传, 2009, 31 ( 5 ) : 515 －522．
[7] 乐佩琦, 陈宜瑜．中国濒危动物红皮书－鱼类[M]．北京: 科学出版社, 1998: 29 － 31 ．
[8] 董崇智, 李怀明 , 赵春刚．哲罗鱼分布区域及其变化 [J]．水产学杂志, 1998, 11 (1 ) : 65 － 70．
[9] 任慕莲, 郭焱, 张秀善．中国额尔齐斯河鱼类资源及渔业[M]．乌鲁木齐: 新疆科技卫生出版社, 2002: 58 －63．
[10] 姜作发, 尹家胜, 伟徐．人工养殖条件下哲罗鱼生长的初步研究[J]．水产学报, 2003, 27 ( 6 ) : 590 －595．
[11] 徐伟, 孙惠武, 关海红．哲罗鱼全人工繁殖的初步研究[J]．中国水产科学, 2007, 14(6) : 896 － 902．
[12] Holcik J, Hensel K, Nieslanik J．The eurasian huchenhucho hucho: Largest salmon of the world[M]．Hingham( USA) : Kluwer Academic Publishers, 1988: 1 － 131 ．
[13] 佟广香, 鲁翠云, 匡友谊, 等．哲罗鱼基因组微卫星富集文库的构建与分析[J]．中国水产科学, 2006, 13(2) : 181 － 186．
[14] Bostein D, White R L, Sckolnick M, et al．Construction ofa genetic linkage map in man using restriction fragmentlength polymorphisms[J]．Am J Hum Genet, 1980, 32:317 － 331 ．
[15] Butterwerth A, Reeves N, HaIb0ur D, et al．Moleculartyping of strains of staphylococcus aureus isolated frombone and joint lesions in lame burlier by random amplification of polymorphic DNA[J]．Poulty Sci, 2001 , 80:1339 － 1343．
[16] 佟广香, 匡友谊, 许凌雪, 等．哲罗鲑形态性状与体重的相关性分析[J]．水产学杂志, 2011 , 24 ( 2) : 31 －36．
[17] 佟雪红, 董在杰, 缪为民, 等．建鲤与黄河鲤的杂交优势研究及主要生长性状的通径分析[J]．大连水产学院学报, 2007, 22(3) : 159 － 164．
[18] 于飞, 张庆文, 孔杰, 等．大菱鲆测量性状对体重的影响效果分析[J]．海洋水产研究, 2008, 29(6) : 33－ 40．
[19] 刘贤德, 蔡明夷, 王志勇 , 等．闽－粤东族大黄鱼生长性状的相关与通径分析[J]．中国海洋大学学报，2008, 38(6) : 916 － 920．
[20] 孙效文, 鲁翠云, 匡友谊, 等．分子标记指导的镜鲤育成品种的遗传结构估化[J]．发明专利公报, 2008, 24(7) : 15 － 21 ．
[21] 储明星, 王吉振, 王爱国 , 等．小尾寒羊五个微卫星基因座遗传多态性研究[J]．遗传学报, 2002, 29 ( 6 ) :502 － 506．
[22] 王得前, 陈国宏, 吴信生, 等．运用微卫星技术分析中国地方鸡品种的亲缘关系[J]．扬州大学学报: 农业与生命科学版, 2003, 24(2) : 1 － 6．
[23] 董秋芬, 刘楚吾, 郭昱嵩, 等．9 种石斑鱼遗传多样性和系统发生关系的微卫星分析[J]．遗传, 2007, 29(7) : 837 － 843．
[24] 匡友谊, 佟广香, 徐伟, 等．黑龙江流域哲罗鲑的遗传结构分析[J]．中国水产科学, 2010, 17 (6) : 1208 －1217．
[25] 黄磊, 王义权．扬子鳄种群的微卫星 DNA 多态及其遗传多样性保护对策分析[J]．遗传学报, 2004, 31(2) : 143 － 150．
[26] 佟广香, 匡友谊, 尹家胜．野生哲罗鱼种质资源遗传多样性的 AFLP 分析[J]．中国水产科学, 2009, 16(6) : 833 － 841 ．

选择文件类型/文献管理软件名称

选择包含的内容