昼间亚高温影响番茄叶片光合及PSⅡ活性的钙素调控作用

doi:10.7668/hbnxb.2011.03.030

摘要/Abstract

摘要： 为分析番茄叶片光合作用及光系统Ⅱ(PS Ⅱ)活性对亚高温逆境响应的钙素调控作用.试验以番茄(Lycopersicon esculentum Mill.)辽园多丽为试材,研究了GaCl₂、EGTA(钙螯合剂)、LaCl3(钙通道阻塞剂)预处理对亚高温胁迫下番茄叶片的叶绿素含量、净光合速率及叶绿素荧光参数的影响.结果表明:昼间亚高温显著抑制了番茄叶片的净光合速率(Pn),降低了叶绿素含量、光系统Ⅱ最大光化学效率(Fv/Fm)、实际光化学量子效率(φPSⅡ)、电子传递速率(ETR)和光化学猝灭系数(qP),提高了初始荧光(Fo)和非光化学猝灭系数(NPQ),对气孔导度(Gs)、蒸腾速率(Tr)、气孔限制值(Ls)有一定的影响;增施CaCl₂显著提高了亚高温胁迫下番茄叶片的叶绿素含量和Pn,且使Fo、Fy/Fm、φPSⅡ、NPQ均提高到对照水平,ETR和qP提高到接近对照水平,表明增施CaCl₂可缓解亚高温对番茄叶片PS Ⅱ活性的影响,但对Ls和胞间CO₂浓度(Ci)影响不大;增施钙抑制剂(EGTA、LaCl₃)进一步加重了亚高温胁迫对番茄叶片PSⅡ的伤害.昼间亚高温降低番茄叶片光合速率与气孔因素无关,可能主要是通过伤害PSⅡ所导致的PSⅡ活性下降所致;而增施CaCl₂可以缓解亚高温对PSⅡ的伤害,维持PSⅡ较高的活性,从而缓解亚高温对番茄净光合速率的影响.

关键词: 番茄, 亚高温, 钙, 叶绿素含量, 光合作用, PSⅡ活性

Abstract: To analyze the regulation of calcium on photosynthesis and PS Ⅱ activity in tomato leaves under sub-high temperature stress.During the period of sub-high temperature,tomatoes liaoyuanduoli grown in the plastic pot were pretreated with CaCl₂,EGTA(Ca2+ chelator),and LaCl₃(Ca2+ channel inhibitor).Chlorophyll content,photosynthetic rate and chlorophyll fluorescence parameters were investigated.The results showed that photosynthesis in tomato leaves pretreated with daytime sub-temperature was significantly inhibited tomato,and chlorophyll content,Fv/Fm,ΦPS Ⅱ,ETR and qP decreased,but Fo and NPQ increased.Furthermore there were some influences on Gs,Tr and Ls;Chlorophyll content and net photosynthesis rate(Pn) in tomato leaves pretreated with CaCl₂ were significantly increased,and there was no difference in the Fo,Fv/Fm,ΦPS Ⅱ and NPQ between CaCl₂ pretreatment and control,while qP,ETR was slightly lower than that of control,these results showed that there was high PS Ⅱ activity in tomato leaves pretreated with CaCl₂,however,Ls and Ci were not affected;The PS Ⅱ activity in tomato leaves pretreated with calcium inhibitor suffered more serious injuries.Inhibition of photosynthesis in daytime sub-high temperature stress had no relation with stomatal factors,the reduction of photosynthesis may be deceased PS Ⅱ activity which was caused by the damage of PS Ⅱ.CaCl₂ pretreatment effectively alleviated the damage to the function of PS Ⅱ in tomato leaves under sub-high temperature stress,and maintained higher PS Ⅱ activity.Consequently CaCl₂ can relieve the inhibition of photosynthesis due to daytime sub-high temperature stress.

Key words: Tomato, Sub-high temperature, Calcium, Chlorophyll content, Photosynthesis, PS Ⅱ activity

中图分类号:

S641.2

李天来, 赵乐, 华利静, 赵旭, 段敏敏. 昼间亚高温影响番茄叶片光合及PSⅡ活性的钙素调控作用[J]. 华北农学报, 2011, 26(3): 150-156. doi: 10.7668/hbnxb.2011.03.030.

LI Tian-lai, ZHAO Le, HUA Li-jing, ZHAO Xu, DUAN Min-min. Regulation of Calcium on Photosynthesis and PS Ⅱ Activity of Tomato Leaves Under Daytime Sub-High Temperature Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 150-156. doi: 10.7668/hbnxb.2011.03.030.

http://www.hbnxb.net/CN/Y2011/V26/I3/150

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