稻瘟病抗病基因<em>Pita</em>和<em>Pib</em>在中国水稻地方品种中的分布

doi:10.7668/hbnxb.2011.03.001

华北农学报 ›› 2011, Vol. 26 ›› Issue (3): 1-6. doi: 10.7668/hbnxb.2011.03.001

所属专题： 水稻；生物技术；

• 论文 • 下一篇

稻瘟病抗病基因Pita和Pib在中国水稻地方品种中的分布

杨杰¹, 杨金欢^1,2, 王军¹, 范方军¹, 朱金燕¹, 曹卿^1,2, 田胜尼², 仲维功¹

1. 江苏省农业科学院粮食作物研究所江苏省优质水稻工程技术研究中心心, 江苏南京 210014;
2. 安徽农业大学生命科学院, 安徽合肥 710058

收稿日期:2011-02-09 出版日期:2011-06-28
通讯作者: 仲维功(1954- )，男，江苏兴化人，研究员，主要从事水稻遗传育种方面的研究。
作者简介:杨杰(1969- )，男，四川南部人，副研究员，博士，主要从事水稻遗传育种方面的研究。杨金欢与第一作者同等贡献。
基金资助:
国家转基因生物新品种培育科技重大专项(2009ZX08001-005B,019B);国家科技支撑计划重大项目(2006BADO2A03);江苏省自然科学基金项目(BK2009321):江苏省农业科技自主创新基金项目;江苏省六大人才高峰项目;江苏省农业科学院基金项目(61107036510806)

Distribution of Two Blast Resistant Genes Pita and Pib in Landrace Rice in China

YANG Jie¹, YANG Jin-huan^1,2, WANG Jun¹, FAN Fang-jun¹, ZHU Jin-yan¹, CAO Qing^1,2, TIAN Sheng-ni², ZHONG Wei-gong¹

1. Institute of Food Crops,Jiangsu Academy of Agriculture Sciences,Jiangsu High Quality Rice R & D Center,Nanjing 210014,China;
2. College of Life Science,Anhui Agricultural University,Hefei 710085,China

Received:2011-02-09 Published:2011-06-28

摘要/Abstract

摘要： 主效抗稻瘟病基因Pita和Pib在我国很多稻区表现高水平的稻瘟病抗性,被广泛应用于我国的水稻育种和生产。但这2个基因在国内品种资源中的分布及利用情况缺乏详细的资料,致使育种利用上存在着盲目性。本研究利用Pita和Pib基因的功能标记,检测和分析了我国115份水稻地方品种的Pita和Pib基因型。结果表明,Pita在我国浙江、福建、广东、广西、贵州、四川、安徽、江西、河南、河北、吉林均有分布,在江苏、上海、山东、湖南、湖北、辽宁没有发现;而只有来自四川的地方品种桂东籼和来自河南的德国稻携带Pib,其他省均未发现。在江苏的主要推广粳稻品种中,晚粳品种几乎都携带这两个基因,部分迟熟中粳携带这两个基因,中熟中粳多数不携带。

关键词: 水稻, 稻瘟病, 主栽品种, 抗性基因, 分子标记

Abstract: The two major blast resistance genes,Pita and Pib,showed broad resistance spectrum to Magnaporthe grisea isolates in many rice planting areas of China and has been utilized in rice breeding and commercial production.However,the distribution of this two genes in present rice germplasms has not been well investigated,resulting in the blindness of their utilization in rice breeding.In the present study,the existence of the two genes in 115 landrace rice germlpasms and 19 rice leading cultivars were rapidly determined using the functional markers of Pita/pita and Pib/pib alleles.The results showed that 20 landrace accessions harbored Pita resistant allele and only 2 landrace accessions harbored Pib resistant allele.The Pita resistant allele distribute widely in china including Zhejiang Province,Fujian Province,Guangdong Province,Guangxi Province,Sichuan Province,Guizhou Province,Anhui Province,Jiangxi Province,Henan Province,Hebei Province,Jilin Province,but not found in Jiangsu Province,Shandong Province,Hunan Province,Hubei Province and Shanghai City.Only two landrace harbored the Pib resistant allele.However,11 cultivars harbored Pita resistant allele and 8 cultivars harbored Pib resistant allele.Interestingly,the 8 cultivars harboring Pib carried the Pita resistant allele simultaneously.These results are useful for rational utilization and deployment of the rice cultivars and germplasm with the Pita and Pib genes and for incorporating and stacking these genes into elite present leading cultivars and lines by marker assisted selection in rice breeding programs nationwide.

Key words: Rice, Rice blast, Leading cuhivar, Resistance gene, DNA marker

中图分类号:

杨杰, 杨金欢, 王军, 范方军, 朱金燕, 曹卿, 田胜尼, 仲维功. 稻瘟病抗病基因Pita和Pib在中国水稻地方品种中的分布[J]. 华北农学报, 2011, 26(3): 1-6. doi: 10.7668/hbnxb.2011.03.001.

YANG Jie, YANG Jin-huan, WANG Jun, FAN Fang-jun, ZHU Jin-yan, CAO Qing, TIAN Sheng-ni, ZHONG Wei-gong. Distribution of Two Blast Resistant Genes Pita and Pib in Landrace Rice in China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(3): 1-6. doi: 10.7668/hbnxb.2011.03.001.

http://www.hbnxb.net/CN/Y2011/V26/I3/1

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稻瘟病抗病基因Pita和Pib在中国水稻地方品种中的分布

Distribution of Two Blast Resistant Genes Pita and Pib in Landrace Rice in China

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稻瘟病抗病基因Pita和Pib在中国水稻地方品种中的分布

Distribution of Two Blast Resistant Genes Pita and Pib in Landrace Rice in China

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