摘要： 从血液氨基酸及生化指标的层面分析日粮添加DDGS对奶牛机体代谢的影响,从而为DDGS在奶牛上的应用提供科学指导。试验选取48头泌乳中期荷斯坦奶牛(DIM(99±58.6)d),按照产奶量、胎次、泌乳日龄相近原则随机分为4组,分别为对照组玉米豆粕日粮(Ctrl)、13.3%的DDGS(DDGS1)、25.1%的DDGS(DDGS2)和34.1%的DDGS(DDGS3),每组12头。在试验第1,4,7周采集尾动脉血样,测定氨基酸组成和生化指标,如血糖、血尿氮、非酯化脂肪酸、胰岛素、谷氨酸脱氢酶等。随着日粮中DDGS比例的增加,血浆亮氨酸含量显著升高,赖氨酸有下降的趋势,必需氨基酸和总氨基酸呈先升高后降低趋势;血尿氮的含量显著降低(P ＜;0.01),非酯化脂肪酸、谷氨酸脱氢酶呈先增加后降低的趋势,血清中甘油三酯的含量有降低的趋势,然而未达到显著水平;DDGS的添加引起谷丙转氨酶的显著降低,DDGS1、DDGS2和DDGS3均低于Ctrl组(P<0.05),其他血液指标在血清中的含量均无差异(P>0.05)。结果表明,日粮中添加DDGS未对奶牛的代谢产生不良影响,然而考虑到DDGS氨基酸组成相对单一,适宜的添加量约25%。

关键词: DDGS, 奶牛, 氨基酸, 血液生化指标

Abstract: To provide scientific sound assistant for the use of dried distillers grains with solubles(DDGS) in dairy cow,we conducted the experiment to study the effect of feeding DDGS on the mechanism of Chinese Holstein Cows through the analysis of blood amino acids and blood metabolites. Forty-eight multiparous Chinese Holstein cows(DIM(99 ± 58. 6) d) were randomly assigned to one of 4 treatments: corn-soybean meal(control,Ctrl), 13. 3% DDGS(DDGS1), 25. 1% DDGS(DDGS2), and 34. 1% DDGS(DDGS3). Blood samples were collected at wk 1,4 and 7 from individual cows from the coccygeal artery. The samples were analyzed for amino acids and blood metabolites.Results showed that Leu in blood plasma increased as the concentration of DDGS increased. Lys tended to decrease following the increased level of DDGS. Essential amino acids and total amino acids in blood plasma had a tendency of increasing until the DDGS reached 25% of diets. Blood serum urea decreased with increasing concentrations of dietary DDGS. Blood NEFA and GLDH tended to increase linearly until the percentage of DDGS reached 34. 1% DM,in all treatment DDGS2 was highest. TG in serum had a tendency to decrease,though did not differ significantly.The serum ALT decreased for DDGS1,DDGS2 and DDGS3,compared with Ctrl. The other blood metabolites were similar for all treatments. Results of this study showed that the use of DDGS did not have any negative impact on the metabolism of cow. In concern with the single amino acid composition,it is appropriate for the dairy ration being formulated to contain about 25% DDGS(DM basis).

Key words: DDGS, Dairy cow, Amino acids, Blood metabolites

中图分类号: 

• S823.01