E<sub>2</sub>通过p38 MAPK及PI3K/Akt信号通路调控奶牛子宫内膜上皮细胞中COX-2的表达

doi:10.7668/hbnxb.20191025

摘要/Abstract

摘要： 为了探究p38 MAPK及PI3K/Akt信号通路是否参与雌二醇（E₂）对奶牛子宫内膜上皮细胞中COX-2的表达及PGF_2α分泌的调节，通过体外培养妊娠早期奶牛子宫内膜上皮细胞，并添加E₂单独或联合p38 MAPK抑制剂SB203580和PI3K抑制剂LY294002处理奶牛子宫内膜上皮细胞。通过Western Blot、qRT-PCR检测COX-2基因和蛋白的表达，ELISA技术检测PGF_2α的水平变化。结果显示：E₂促进了妊娠早期奶牛子宫内膜上皮细胞中COX-2的表达和PGF_2α的分泌；与此同时，E₂激活了p38 MAPK及PI3K/Akt信号通路，添加SB203580和LY294002则抑制了E₂对COX-2表达及PGF_2α分泌的促进作用。说明E₂通过激活p38 MAPK及PI3K/Akt信号通路促进了COX-2的表达及PGF_2α的分泌。

关键词: 奶牛, 子宫内膜上皮细胞, 雌二醇, 环氧合酶2, 前列腺素F_2α

Abstract: To investigate whether p38 MAPK and PI3K/Akt signaling pathways are involved in the regulation of estradiol(E₂) on PGF_2α secretion in cow endometrial epithelial cells, endometrial epithelial cells of pregnant dairy cows were cultured in vitro, and different concentrations of E₂ and p38 MAPK and PI3K/Akt pathway inhibitors SB 203580 and LY294002 were used to treat cows' endometrial epithelial cells respectively. The expression of COX-2 gene and protein and the level of PGF_2α were detected by Western Blot, qRT-PCR and ELISA. The results showed that E₂ promoted the expression of COX-2 and the secretion of PGF_2α. In addition, E₂ activated the p38 MAPK and PI3K/Akt signaling pathways. Moreover, the addition of SB203580 and LY294002 inhibit the promotion effect of E₂ on COX-2 expression and PGF_2α secretion. Therefore, it proved that E₂ promoted the expression of COX-2 and the secretion of PGF_2α by activating the p38 MAPK and PI3K/Akt signaling pathways.

Key words: Cow, Endometrial epithelial cells, Estradiol, Cyclooxygenase-2, Prostaglandin F_2α

中图分类号:

S795.7

封紫嫣, 王相国, 袁梦仪, 付博凡, 谢彤彤, 常迪, 吴春阳, 倪和民, 肖龙菲. E₂通过p38 MAPK及PI3K/Akt信号通路调控奶牛子宫内膜上皮细胞中COX-2的表达[J]. 华北农学报, 2021, 36(1): 232-238. doi: 10.7668/hbnxb.20191025.

FENG Ziyan, WANG Xiangguo, YUAN Mengyi, FU Bofan, XIE Tongtong, CHANG Di, WU Chunyang, NI Hemin, XIAO Longfei. E₂ Regulates the Expression of COX-2 in Endometrial Epithelial Cells of Cows Through p38 MAPK and PI3K/Akt Signaling Pathway[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 232-238. doi: 10.7668/hbnxb.20191025.

http://www.hbnxb.net/CN/Y2021/V36/I1/232

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E₂通过p38 MAPK及PI3K/Akt信号通路调控奶牛子宫内膜上皮细胞中COX-2的表达

E₂ Regulates the Expression of COX-2 in Endometrial Epithelial Cells of Cows Through p38 MAPK and PI3K/Akt Signaling Pathway

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E2通过p38 MAPK及PI3K/Akt信号通路调控奶牛子宫内膜上皮细胞中COX-2的表达

E2 Regulates the Expression of COX-2 in Endometrial Epithelial Cells of Cows Through p38 MAPK and PI3K/Akt Signaling Pathway

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E₂通过p38 MAPK及PI3K/Akt信号通路调控奶牛子宫内膜上皮细胞中COX-2的表达

E₂ Regulates the Expression of COX-2 in Endometrial Epithelial Cells of Cows Through p38 MAPK and PI3K/Akt Signaling Pathway