脲酶B亚单位疫苗免疫对奶牛血清蛋白质组分的影响

doi:10.3969/j.issn.1000-7091.2012.05.037

华北农学报 ›› 2012, Vol. 27 ›› Issue (5): 198-202. doi: 10.3969/j.issn.1000-7091.2012.05.037

所属专题： 畜牧；

脲酶B亚单位疫苗免疫对奶牛血清蛋白质组分的影响

袁廷杰^1,2, 王加启², 杨永新², 卜登攀², 章玉涛², 赵圣国², 周凌云², 李发弟¹

甘肃农业大学动物科学技术学院;
中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室

收稿日期:2012-07-25 出版日期:2012-10-28
通讯作者: 王加启(1967-)，男，安徽宿州人，研究员，博士生导师，主要从事反刍动物营养与牛奶质量改良研究。
作者简介:袁廷杰(1986-)．男，河北曲周人，硕士研究生，主要从事反刍动物营养研究。
基金资助:
北京市自然科学基金资助项目(6092017)

Effect of Ure B Subunit Vaccine Immunization on Serum Proteins in Dairy Cows

YUAN Ting-jie^1,2, WANG Jia-qi², YANG Yong-xin², BU Deng-pan², ZHANG Yu-tao², ZHAO Sheng-guo², ZHOU Ling-yun², LI Fa-di¹

1. Faculty of Animal Science & Technology Gansu Agricultural University Lanzhou 730070 China ;

2. State Key Laboratory of Animal Nutrition Institute of Animal Sciences Chinese Academy of Agriculture Sciences Beijing 100193 China

Received:2012-07-25 Published:2012-10-28

摘要/Abstract

摘要： 为探讨脲酶B(Ure B)亚单位疫苗免疫后奶牛血清蛋白质组分的表达变化。选用8头健康的泌乳荷斯坦奶牛,分为免疫处理组和空白对照组,分别于胯部两侧肌肉注射Ure B亚单位疫苗(含抗原蛋白0.2 mg)和生理盐水,每隔2周进行加强免疫,并于免疫后第56天采集奶牛血样,用间接ELISA法测定血清中特异性IgG、IgM和IgA效价,用二维凝胶电泳(2-DE)结合MALDI-TOF-TOF质谱技术研究血清蛋白质组表达变化。免疫处理组奶牛血清中特异性IgG效价显著高于对照组(P<0.05),但Ure B免疫对奶牛血清中特异性IgM和IgA效价无显著影响(P>0.05)。血清蛋白质组分析发现6个差异表达蛋白点,其中3个蛋白点得到有效鉴定。结合珠蛋白前体和其他3个蛋白点在免疫Ure B亚单位疫苗奶牛血清中表达量增加,而Kakapo蛋白的表达量降低。Ure B亚单位疫苗免疫引起奶牛血清中结合珠蛋白和Kakapo等蛋白表达发生变化,为进一步研究脲酶B亚单位疫苗免疫对奶牛机体的影响提供了依据。

关键词: 脲酶B亚单位疫苗, 奶牛, 血清, 蛋白质组

Abstract: This study was to investigate the changes of serum proteins in dairy cows immunized with Ure B subunit vaccine.Eight healthy cows were divided into 2 groups and immunized with 0.2 mg antigen protein or saline by intramuscular injection on both sides of the hip,once every two weeks,and total 4 times for immunization.Serum samples were collected on 56 d after immunization with Ure B subunit vaccine.Specific antibody in blood samples were determined by ELISA methods.Serum proteomic analysis were performed using two-dimensional gel electrophoresis(2-DE) coupled with MALDI-TOF-TOF mass spectrometer.Specific IgG were significantly higher after the cows immunized with Ure B subunit vaccine than control groups(P0.05),whereas IgM and IgA was not significant(P0.05).Proteomic analysis revealed 6 protein spots were differentially expressed,and 3 spots were successfully identified.Haptoglobin precursor and 3 unknown spots were up-regulated in cows immunized with Ure B subunit vaccine,while Kakapo was down-regulated.The current study showed that cows injection with Ure B subunit vaccine can result in the change of haptoglobin,Kakapo and other proteins,which provide the basis for further research of the physiology effects of Ure B subunit vaccine on dairy cows.

Key words: Ure B subunit vaccine, Dairy cow, Scrum Proteome

中图分类号:

S823

袁廷杰, 王加启, 杨永新, 卜登攀, 章玉涛, 赵圣国, 周凌云, 李发弟. 脲酶B亚单位疫苗免疫对奶牛血清蛋白质组分的影响[J]. 华北农学报, 2012, 27(5): 198-202. doi: 10.3969/j.issn.1000-7091.2012.05.037.

YUAN Ting-jie, WANG Jia-qi, YANG Yong-xin, BU Deng-pan, ZHANG Yu-tao, ZHAO Sheng-guo, ZHOU Ling-yun, LI Fa-di. Effect of Ure B Subunit Vaccine Immunization on Serum Proteins in Dairy Cows[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 198-202. doi: 10.3969/j.issn.1000-7091.2012.05.037.

http://www.hbnxb.net/CN/Y2012/V27/I5/198

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Effect of Ure B Subunit Vaccine Immunization on Serum Proteins in Dairy Cows

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