日粮精粗比对围产期奶牛乳腺内参基因表达的影响

doi:10.3969/j.issn.1000-7091.2013.04.014

华北农学报 ›› 2013, Vol. 28 ›› Issue (4): 75-78. doi: 10.3969/j.issn.1000-7091.2013.04.014

所属专题： 畜牧；生物技术；

日粮精粗比对围产期奶牛乳腺内参基因表达的影响

秦彤, 王皓宇, 郝海生, 王栋, 杜卫华, 朱化彬

中国农业科学院北京畜牧兽医研究所, 北京 100193

收稿日期:2013-05-15 出版日期:2013-08-28
通讯作者: 朱化彬(1965－ )，男，安徽蚌埠人，博士，研究员，主要从事奶牛胚胎生物技术的研究
作者简介:秦彤(1980－ )，女，河北石家庄人，博士，助理研究员，主要从事奶牛泌乳调控研究
基金资助:
国家“973”计划项目(2011CBl00805);中央级公益性科研院所基本科研业务费专项资金项目(2011cj-10-1)

Reference Gene Expression in Mammary Tissue of Periparturient Cows Is Affected by Dietary Forage to Concentrate Ratio

QIN Tong, WANG Hao-yu, HAO Hai-sheng, WANG Dong, DU Wei-hua, ZHU Hua-bin

Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2013-05-15 Published:2013-08-28

摘要/Abstract

摘要： 选择合适的内参基因是利用实时荧光定量PCR准确分析基因相对表达量的先决条件。选择了奶牛乳腺研究中广泛使用的7个候选内参基因,包括GAPDH、ACTB、18S rRNA、RPS9、RPS15、UXT、MYC。奶牛以精粗比不同的2种日粮饲喂,并在产后16 d采集乳腺组织。利用qRT-PCR技术探讨了候选内参基因在围产奶牛乳腺组织中的表达情况,并利用geNorm程序对数据进行分析。结果发现,UXT和RPS9表达稳定,不受日粮精粗比的影响,因此,可作为围产期奶牛乳腺组织基因表达分析中相对合适的内参基因。

关键词: 围产奶牛, 乳腺, 内参基因, 实时荧光定量PCR, geNorm程序

Abstract: Selection of appropriate reference genes for normalization of quantitative PCR data for genes of inter- est is critical for interpretation of results．Seven candidate reference genes including GAPDH，ACTB，18S rRNA， RPS9，RPS15，UXT and MYC were selected in this research．Cows were fed different forage to concentrate(F: C) ra- tio dietary and mammary biopsies were taken at 16 day postpartum．The mRNA expression levels of seven candidate reference genes in mammary tissue were detected by qRT- PCR．Gene copy number was determined using absolute quantification，and then the expression stability of these genes were analysed by geNorm software．These results sug- gested that UXT and RPS9 showed less variability between the cows and were unaffected by dietary F: C ratio and， therefore，could be used to normalize mRNA levels of mammary in periparturient cows．

Key words: Periparturient cows, Mammary gland, Reference gene, qRT- PCR, geNorm software

中图分类号:

秦彤, 王皓宇, 郝海生, 王栋, 杜卫华, 朱化彬. 日粮精粗比对围产期奶牛乳腺内参基因表达的影响[J]. 华北农学报, 2013, 28(4): 75-78. doi: 10.3969/j.issn.1000-7091.2013.04.014.

QIN Tong, WANG Hao-yu, HAO Hai-sheng, WANG Dong, DU Wei-hua, ZHU Hua-bin. Reference Gene Expression in Mammary Tissue of Periparturient Cows Is Affected by Dietary Forage to Concentrate Ratio[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 75-78. doi: 10.3969/j.issn.1000-7091.2013.04.014.

http://www.hbnxb.net/CN/Y2013/V28/I4/75

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日粮精粗比对围产期奶牛乳腺内参基因表达的影响

Reference Gene Expression in Mammary Tissue of Periparturient Cows Is Affected by Dietary Forage to Concentrate Ratio

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日粮精粗比对围产期奶牛乳腺内参基因表达的影响

Reference Gene Expression in Mammary Tissue of Periparturient Cows Is Affected by Dietary Forage to Concentrate Ratio

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