华北农学报 ›› 2014, Vol. 29 ›› Issue (1): 145-150. doi: 10.7668/hbnxb.2014.01.027

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砍收时期对白肋烟上部叶晾制前后氨基酸含量的影响

孙红恋1, 赵田1, 周海燕1, 孙军伟2, 字萍3, 宋莹丽1, 王瑞云1, 吴疆1, 史宏志1   

  1. 1. 河南农业大学国家烟草栽培生理生化研究基地,河南郑州 450002;
    2. 云南省烟草公司大理州公司,云南大理 671000;
    3. 云南烟草宾川白肋烟有限责任公司,云南宾川 671600
  • 收稿日期:2013-06-20 出版日期:2014-02-28
  • 通讯作者: 史宏志(1963-),男,河南滑县人,教授,博士,主要从事烟草栽培和生理研究.
  • 作者简介:孙红恋(1987-),女,河南扶沟人,在读硕士,主要从事烟草栽培生理研究.
  • 基金资助:
    云南省烟草公司科技项目(2010年YN50)

Effect of Stalk Cutting Time on Upper Leaf Amino Acid Content in Burley Tobacco before and after the Air-curing

SUN Hong-lian1, ZHAO Tian1, ZHOU Hai-yan1, SUN Jun-wei2, ZI Ping3, SONG Ying-li1, WANG Rui-yun1, WU Jiang1, SHI Hong-zhi1   

  1. 1. National Tobacco Cultivation&Physiology&Biochemistry Research Center, Henan Agricultural University, Zhengzhou 450002, China;
    2. Dali Tobacco Company of Yunnan Province, Dali 671000, China;
    3. Binchuan Burley Tobacco Co., Ltd., Binchuan 671600, C hina
  • Received:2013-06-20 Published:2014-02-28

摘要: 以白肋烟TN 86为材料,研究了砍收期对晾制前后上部叶氨基酸含量的影响。结果表明,晾制前总氨基酸含量为124.47~140.78 mg/g,以酪氨酸含量和比例较高,在各个砍收时期天冬氨酸含量为7.78~11.16 mg/g,表现为打顶后4周>6周>5周>3周>2周。晾制前甘氨酸和苏氨酸与砍收时期相关性达显著水平。晾制后总氨基酸含量为73.78~97.77 mg/g,以天冬氨酸比例和含量较高,在各个砍收时期天冬氨酸含量为23.73~31.25 mg/g,以打顶后2周砍收的含量较高,打顶后4周砍收比例较高。天冬氨酸在晾制前含量和比例不高,晾制后却大幅度上升。晾制后甘氨酸、苏氨酸、脯氨酸、异亮氨酸、亮氨酸、酪氨酸、苯丙氨酸和赖氨酸与砍收期相关性达到极显著水平,天冬氨酸、组氨酸、精氨酸、丙氨酸、缬氨酸、蛋氨酸和总氨基酸与砍收期的相关性达到显著水平。砍收时期对晾制后氨基酸含量影响大于晾制前。打顶后2周砍收有利于上部叶天冬氨酸积累和形成。

关键词: 白肋烟, 上部叶, 砍收时期, 氨基酸, 晾制

Abstract: This paper studied effect of different stalk cutting time on upper leaves amino acid content before and after the air-curing of burley tobacco TN 86. The results showed that,the total acids content was in the range of 124. 47- 140. 78 mg / g,the content and ratio of Tyr were higher before the air-curing,the Asp acid content was in the range of 7. 78- 11. 16 mg / g at different stalk cutting time before the air-curing,manifested as topping four weeks six weeks five weeks three weeks two weeks. There was a significant level between Gly,Thr and stalk cutting time before the air-curing. The total acids content was in the range of 73. 78- 97. 77 mg / g,the content and proportion of Asp were higher after the air-curing. The Asp acid content was in the range of 23. 73- 31. 25 mg / g at different stalks cutting time after the air-curing; its content received a higher proportion when stalk cutting after topping two weeks and its ratio received a higher proportion when stalk cutting after topping four weeks. The content and the ratio of Asp were not high before the air-curing,but increased significantly after the air-curing. There was a extremely significant level between Gly,Thr,Pro,Ile,Leu,Lys,Phe,Tyr and stalk cutting time; there was a significant level between Asp,His,Arg,Ala,Val,Met,total amino acids and stalk cutting time. Effect of the amino acid contents after air-curing was greater than before air-curing at different stalk cutting time. It was conducive to the accumulation and formation of Asp acid in upper leaves when stalk cutting after topping two weeks.

Key words: Burley tobacco, Upper leaves, Stalk cutting time, Amino acid, Air-curing

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引用本文

孙红恋, 赵田, 周海燕, 孙军伟, 字萍, 宋莹丽, 王瑞云, 吴疆, 史宏志. 砍收时期对白肋烟上部叶晾制前后氨基酸含量的影响[J]. 华北农学报, 2014, 29(1): 145-150. doi: 10.7668/hbnxb.2014.01.027.

SUN Hong-lian, ZHAO Tian, ZHOU Hai-yan, SUN Jun-wei, ZI Ping, SONG Ying-li, WANG Rui-yun, WU Jiang, SHI Hong-zhi. Effect of Stalk Cutting Time on Upper Leaf Amino Acid Content in Burley Tobacco before and after the Air-curing[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 145-150. doi: 10.7668/hbnxb.2014.01.027.

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