苹果茎尖组织中GA2ox基因的结构特征及蛋白序列的生物信息学分析

doi:10.3969/j.issn.1000-7091.2012.05.011

华北农学报 ›› 2012, Vol. 27 ›› Issue (5): 55-59. doi: 10.3969/j.issn.1000-7091.2012.05.011

所属专题： 苹果；生物技术；

苹果茎尖组织中GA2ox基因的结构特征及蛋白序列的生物信息学分析

白牡丹, 王彩虹, 田义轲, 陈宝印, 刘云龙

青岛农业大学园林园艺学院

收稿日期:2012-05-10 出版日期:2012-10-28
通讯作者: 王彩虹(1968-)，女，陕西礼泉人，博士，硕士生导师，主要从事果树生物技术与遗传育种研究。
作者简介:白牡丹(1988-)，女，山西柳林人，在读硕士，主要从事果树生物技术与遗传育种研究。
基金资助:
山东省良种工程项目;青岛市科技计划基础研究项目(11-2-4-5(4)-jch)

Gene Structure Identification and Protein Sequence Bioinformation Analysis for GA2ox from Apical Tissue of Apple Shoots

BAI Mu-dan, WANG Cai-hong, TIAN Yi-ke, CHEN Bao-yin, LIU Yun-long

College of Landscape and Horticulture Qingdao Agricultural University Qingdao 266109 China

Received:2012-05-10 Published:2012-10-28

摘要/Abstract

摘要： GA2ox是赤霉素合成代谢的关键酶,以苹果品种富士茎尖为供试材料,应用同源克隆技术克隆GA2ox基因,获得长度为1 656 bp的DNA序列和1 014 bp cDNA序列。对比分析表明,DNA序列存在3个外显子和2个内含子。GA2ox的开放性阅读框编码337个氨基酸残基,推断其相对分子量为37.679 8 kDa,等电点为6.41。蛋白质结构域检索表明,GA2ox属于依赖于2-酮戊二酸的双加氧酶家族成员;氨基酸同源性分析表明,GA2ox与已报道的其他植物的GA2ox氨基酸序列同源性为51%～97%;氨基酸聚类分析表明,苹果和梨首先聚类,其次是毛果杨。

关键词: 苹果, GA2ox, 保守结构域, 氨基酸同源性分析, 氨基酸聚类分析

Abstract: GA 2-oxidase(GA2ox)is a critical enzyme in the pathway of gibberellins biosynthesis.In this research,GA2ox gene was isolated from Fuji stem apical tissue by homologous cloning technology.The cDNA sequence of GA2ox was 1 656 bp in length with 3 exons and 2 introns.Its open reading frame was 1 014 bp which encoding a protein of 337 amino acids.Molecular weight and isoelectric point of the protein was 37.679 8 kDa and 6.41,respectively.Conserved domains showed that the enzyme belonged to the class 2-oxoglutarate-dependent dioxygenases.Amino acids homology analysis indicated that the sequence had 51%-97% similarity with those of other reported plants.Cluster analysis showed that GA2ox from pear was clustered together with GA2ox from apple firstly,and followed by that from black cottonwood.

Key words: Apple(Malus domestica), GA2ox, Conserved domains, Amino acid homologous analysis, Cluster analysis

中图分类号:

S661.1

白牡丹, 王彩虹, 田义轲, 陈宝印, 刘云龙. 苹果茎尖组织中GA2ox基因的结构特征及蛋白序列的生物信息学分析[J]. 华北农学报, 2012, 27(5): 55-59. doi: 10.3969/j.issn.1000-7091.2012.05.011.

BAI Mu-dan, WANG Cai-hong, TIAN Yi-ke, CHEN Bao-yin, LIU Yun-long. Gene Structure Identification and Protein Sequence Bioinformation Analysis for GA2ox from Apical Tissue of Apple Shoots[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 55-59. doi: 10.3969/j.issn.1000-7091.2012.05.011.

http://www.hbnxb.net/CN/Y2012/V27/I5/55

参考文献

[1] 田义轲.苹果柱型基因作图及贝壳杉烯氧化酶基因cDNA片段克隆[D].西安:西北农林科技大学,2004.

[2] Carrea E, Bou J, Garcia-Martinez J, et al. Changes in GA 20-oxidase gene expression strongly affect stem length, tube induction and tuber yield of potato plants [J]. Plant J,2000,22:247 256.

[3] Sakamoto T, Miura K, Itoh H ,et al. An overview of gibberellins metabolism enzyme genes and their related mutants in rice[J]. Plant physiol,2004,134 : 1642 1653.

[4] Dellaporta S L, Calderon-Urrea A. Sex determination in flowering plants [J]. Plant Cell, 1993,5 : 1241 1251.

[5] Davies P J. Plant hormones: physiology, biochemistry and molecular biology [M]. Dordrecht, The Netherlands : Kluwer Academic Publishers, 1995,207 ( 16 ) : 852 867.

[6] Yaxley J R, Ross J J, Sherriff L J, et al. Gibberellin biosynthesis mutations and root development in pea [J]. Plant Physiol,2001,125 ( 14 ) :621 633.

[7] Ogas J, Cheng J C,Sung Z R,et al. Cellular differentiation regulated by gibberellin in the Arabidopsis thaliana pickle mutant[J]. Science, 1997,277:91 94.

[8] Hedden P, Phillips A L. Gibberellin metabolism:new insights revealed by the genes [J]. Trends Plant Sci,2000, 5:523 530.

[9] Graebe J E. Gibberellin biosynthesis and control[J]. Annu Rev Plant Physiol, 1987,38:419 465.

[10] Olszewski N, Sun T P, Gubler F. Gibberellin signaling : Biosynthesis, catabolism, and response pathways [J]. Plant Cell ,2002,14:61 80.

[11] Yamaguchi S. Gibberellin metabolism and its regulation [J]. Annu Rev Plant Biol,2008,59:225 251.

[12] MacMillan J. Biosynthesis of the gibberellin plant hormones [J]. Nat Prod Rep, 1997,14:221 243.

[13] Rieu I,Eriksson S,Powers S J,et al. Genetic analysis reveals that C19-GA 2-oxidatio is a major gibberellin inactivation pathway in Arabidopsis [J]. Plant Cell, 2008, 20:2420 2436.

[14] Thomas S G,Phillips A L, Hedden P. Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation [J]. Proc Natl Acad Sci, 1999,96:4698 4703.

[15] Schomburg F M, Bizzell C M, Lee D J,et al. Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants [J]. Plant Cell ,2003,15 : 151 163.

[16] Lee D J,Zeevaart J A D. Molecular cloning of GA 2-oxidase3 from spinach and its ectopic expression in Nicotiana sylvestris[J]. Plant Physiol,2005 ,138 :243 254.

[17] Zhao H J, Dong J L,Wang T. Function and Expression Analysis of Gibberellin Oxidases in Apple [J]. Plant Mol Biol Rep ,2010,28:231 238.
[18] Wilmouth R C,Turnbull J J,Welford R W, et al. Structure and mechanism of anthocyanidin synthase from Arabidopsis thaliana[J]. Structure ,2002, 10:93 103.

[19] Hedden P,Preebsting W M. Genetic analysis al grbberellin brosynthesis [J]. Plant Physiol, 1999,119 ( 15 ) : 365 370.

[20] Lange T. Molecular biology of gibberellin synthesis [J]. Planta, 1998,204:409 419.＜br＞
[21] Kusaba S, Honda C, Kano M Y. Isolation and expression analysis of gibberellin 20-oxidase homologous gene in apple [J]. Journal of Experimental Botany, 2001, 52 (335) :375 -376.

[22] 石琰琛.果树GA生物合成基因-贝壳杉烯氧化酶和20-氧化酶的克隆及表达模式的初步研究[D].泰安:山东农业大学,2002.

[23] Hytonen T, Elomaa P, Moritz T, et al. Gibberellin mediates day length-controlled differentiation of vegetative meristems in strawberry (Fragaria x ananassa Duch) [J]. BMC Plant Biology,2009,9(18) :1 12.

[24] 宣利利.梨GA20-氧化酶和贝壳杉烯氧化酶基因克隆及表达分析[D].北京:中国农业科学院果树研究所,2011.

选择文件类型/文献管理软件名称

选择包含的内容

苹果茎尖组织中GA2ox基因的结构特征及蛋白序列的生物信息学分析

Gene Structure Identification and Protein Sequence Bioinformation Analysis for GA2ox from Apical Tissue of Apple Shoots

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	贾朝爽, 王志华, 王文辉. 不同货架温度结合1-MCP处理对华红、华月苹果质地性状的影响[J]. 华北农学报, 2022, 37(4): 128-140.
[2]	路晓月, 王子夜, 韩建玮, 许露, 张晓飞, 韩杰, 王志刚, 刘越, 索相敏, 阎爱华. 木醋液对八棱海棠生长及再植病土壤主要特性的影响[J]. 华北农学报, 2022, 37(3): 175-185.
[3]	赵惠英, 杨光凯, 高燕, 张小军, 郝燕燕. *苹果MdSGR2基因克隆及植物超表达载体构建*[J]. 华北农学报, 2022, 37(2): 42-48.
[4]	张涛, 张林, 周剑光, 甘金华, 何力. 巨须裂腹鱼5种组织乳酸脱氢酶和苹果酸脱氢酶的电泳分析[J]. 华北农学报, 2021, 36(S1): 390-396.
[5]	王骞, 蔡华成, 王淑婷, 张小军, 高敬东, 杜学梅, 李春燕, 郝燕燕, 杨廷桢. 高纺锤树形下2个中间砧富士苹果生长与叶片光合特性的比较[J]. 华北农学报, 2021, 36(1): 108-115.
[6]	付静, 孙梦, 马春晖, 黄永红. 4种葱属植物代谢组分析及其主要成分对苹果轮纹病菌的抑制作用[J]. 华北农学报, 2020, 35(S1): 363-370.
[7]	高源, 王大江, 王昆, 李连文, 朴继成. 基于叶绿体片段序列的苹果属植物遗传多样性[J]. 华北农学报, 2020, 35(6): 42-51.
[8]	李世军, 尹宝颖, 李佳, 李中勇, 张学英, 徐继忠. 早熟苹果果实软化过程中乙烯、相关酶及其基因表达变化[J]. 华北农学报, 2019, 34(6): 104-109.
[9]	张朝红, 陈东玫, 杨凤秋, 赵同生, 李扬, 赵国栋, 赵永波. 苹果SLAF图谱构建及果锈基因QTL分析[J]. 华北农学报, 2019, 34(5): 37-44.
[10]	张涛, 陈建武, 何力. 尖裸鲤5种组织乳酸脱氢酶和苹果酸脱氢酶的电泳分析[J]. 华北农学报, 2018, 33(S1): 89-93.
[11]	张海月, 肖玉雄, 田义轲, 王彩虹, 杨绍兰, 李昱, 李玉琪. 一个苹果β-微管蛋白基因及其启动子序列特征分析[J]. 华北农学报, 2018, 33(3): 78-85.
[12]	靳芹, 代培红, 刘超, 胡芹华, 刘晓东, 李月. *四个苹果MdCBF基因克隆与表达分析*[J]. 华北农学报, 2017, 32(S1): 17-22.
[13]	李婷, 练森, 李保华, 梁文星, 王彩霞. 苹果树腐烂病菌β-葡萄糖苷酶基因的克隆及表达分析[J]. 华北农学报, 2017, 32(6): 78-84.
[14]	张国俊, 王婷婷, 胡利宗, 李书粉, 高武军. 苹果Hsf家族成员的序列特征、表达与进化分析[J]. 华北农学报, 2017, 32(2): 71-80.
[15]	苏丽艳, 田爱梅, 陶贵荣, 李蒙. *苹果MdCaM的克隆及其对果实釆后非生物胁迫的响应*[J]. 华北农学报, 2017, 32(1): 47-52.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

苹果茎尖组织中GA2ox基因的结构特征及蛋白序列的生物信息学分析

Gene Structure Identification and Protein Sequence Bioinformation Analysis for GA2ox from Apical Tissue of Apple Shoots

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价