华北农学报 ›› 2014, Vol. 29 ›› Issue (1): 103-107. doi: 10.7668/hbnxb.2014.01.020

所属专题: 小麦 生物技术

• 论文 • 上一篇    下一篇

蓝、紫粒小麦Wx基因分子检测及部分氨基酸含量分析

任文斌1, 谢三刚1, 吴翠翠1, 杨慧勇2, 李素娟3, 杨娜1, 高明1, 宋昱1, 谢飒英1   

  1. 1. 山西省农业科学院棉花研究所,山西运城 044000;
    2. 山西省农业科学院山西晋中 030600;
    3. 山西省农业科学院现代农业研究中心,山西太原高粱研究所 030031
  • 收稿日期:2013-11-13 出版日期:2014-02-28
  • 作者简介:任文斌(1980-),男,山西沁源人,助理研究员,硕士,主要从事小麦遗传育种与栽培研究
  • 基金资助:
    山西省科技攻关项目(20130311001-3);山西省农业科学院育种工程项目(11yzgc102)

Wx Gene Molecular Detection and Statistical Analysis on Part of the Amino Acid Content of Blue or Purple Grain Wheat

REN Wen-bin1, XIE San-gang1, WU Cui-cui1, YANG Hui-yong2, LI Su-juan3, YANG Na1, GAO Ming1, SONG Yu1, XIE Sa-ying1   

  1. 1. Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China;
    2. Sorghum Research Institute, Shanxi Academy of Agricultural Sciences, Jinzhong 030600, China;
    3. Research Center of Modern Agriculture, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
  • Received:2013-11-13 Published:2014-02-28

摘要: 利用3对引物对47份品系进行Wx基因分子检测,其中3份白粒小麦为野生型材料,22份蓝粒小麦和22份紫粒小麦为Wx基因全突变型材料,即全糯小麦。对其部分氨基酸含量进行多重比较,结果表明,总体上蓝粒糯小麦氨基酸含量高于紫粒糯小麦,紫粒糯小麦高于白粒小麦;3种类型小麦精氨酸、半胱氨酸、赖氨酸、蛋氨酸、苏氨酸、缬氨酸含量差异极显著;异亮氨酸、色氨酸含量差异达显著水平。

关键词: 蓝粒小麦, 紫粒小麦, Wx基因, 氨基酸

Abstract: Three primer pairs of Wx gene were used in molecular detection from forty-seven wheat strains. Three white wheats were wild type material,twenty-two Blue grain and twenty-two purple grain of wheats were Wx gene mutation type materials,named the full waxy wheats. Multiple comparative analysis on part of the amino acid content. The results showed that amino acid content of the blue waxy wheats was higher than the purple waxy wheats, the purple waxy wheats was higher than white wheats; Arg,Cys,Lys,Met,Thr,Val content of the three types of wheat were extremely significant different,Ile,Trp content were significantly different.

Key words: Blue gain wheat, Purple gain wheat, Wx gene, Amino acid

中图分类号: 

引用本文

任文斌, 谢三刚, 吴翠翠, 杨慧勇, 李素娟, 杨娜, 高明, 宋昱, 谢飒英. 蓝、紫粒小麦Wx基因分子检测及部分氨基酸含量分析[J]. 华北农学报, 2014, 29(1): 103-107. doi: 10.7668/hbnxb.2014.01.020.

REN Wen-bin, XIE San-gang, WU Cui-cui, YANG Hui-yong, LI Su-juan, YANG Na, GAO Ming, SONG Yu, XIE Sa-ying. Wx Gene Molecular Detection and Statistical Analysis on Part of the Amino Acid Content of Blue or Purple Grain Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 103-107. doi: 10.7668/hbnxb.2014.01.020.

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