中国荷斯坦牛BOLA-DRB3外显子2的PCR-SSCP多态性分析

doi:10.7668/hbnxb.2010.05.020

华北农学报 ›› 2010, Vol. 25 ›› Issue (5): 94-98. doi: 10.7668/hbnxb.2010.05.020

所属专题： 畜牧；

中国荷斯坦牛BOLA-DRB3外显子2的PCR-SSCP多态性分析

王学清¹, 王昆¹, 王沛宇², 李耕³, 孙东晓³, 吴占军¹, 张峰¹

1. 河北省农林科学院, 粮油作物研究所, 河北省农林科学院奶牛研究中心, 河北, 石家庄, 050031;
2. 石家庄市第一中学, 河北, 石家庄, 050019;
3. 中国农业大学, 动物科技学院, 北京, 100094

收稿日期:2010-08-11 出版日期:2010-10-28
作者简介:王学清(1981-),男,河北平山人,助理研究员,硕士,主要从事动物遗传育种研究工作.
基金资助:
河北省自然科学基金项目(C2009001308);河北省农林科学院青年基金项目(2009)资助;河北省农林科学院财政项目(2010055008)

PCR-SSCP Analysis of Genetic Polymorphisms of BoLA-DRB3 Exon 2 Gene in Chinese Holstein

WANG Xueqing¹, WANG Kun¹, WANG Peiyu², LI Geng³, SUN Dongxiao³, WU ZhanjunZHANG Feng1¹

1. Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Dairy Cattle Research Center, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China;

2. Shijiazhuang No. 1 Senior School, Shijiazhuang 050019, China;

3. China Agricultural University, College of Animal Science and Technology, Beijing 100094, China

Received:2010-08-11 Published:2010-10-28

摘要/Abstract

摘要： 通过PCR-SSCP法对中国荷斯坦牛BoLA-DRB3基因的外显子2进行多态性分析.在223个个体中共获得10种等位基因,其中D等位基因频率最高为0.228 7,A等位基因频率最低为0.013 ;等位基因型18种,其中FG基因型频率最高,为0.12 6,其次为HH,基因型频率为0.112 1;ML基因型频率最低,为0.004 .另外分析发现,在不同家系中,DRB3基因外显子2等位基因具有特异性,等位基因的类型和频率存在比较明显的差异.差异显著性分析表明,D等位基因对30 d产奶量和30 d乳蛋白量有明显的负效应,ME基因型个体30 d乳脂量显著低于其他个体.结果表明中国荷斯坦牛的BoLA-DRB3外显子2具有丰富的多态性,并具有家系分布的特异性.

关键词: 中国荷斯坦牛, PCR-SSCP, BoLA-DRB3外显子2, 多态性

Abstract: In the present work,genetic polymorphisms of BoLA-DRB3 * exon2 gene in 223 Chinese Holstein were detected by PCR-SSCP. In total,18 SSCP patterns were identified and the frequency of each pattern within the herd was determined. The frequency of pattern FG was the highest which was 0. 125 6,the next frequency of pattern HH was 0. 112 1,the frequency of pattern ML was the lowest which was 0. 004 5. We found that SSCP patterns of different families were specific. Differences of significance analysis indicates that,D alleles has a significant negative effects on 305 days milk yield and 305 days milk protein content,305 days milk fat content of ME genotype significantly lower than other individuals. Preliminary analysis result revealed high genetic polymorphism of exon2 region of BoLA-DRB3 gene in Chinese Holstein.

Key words: Holstein, PCR-SSCP, BoLA-DRB3 exon2, Polymorphism

中图分类号:

S823.03

王学清, 王昆, 王沛宇, 李耕, 孙东晓, 吴占军, 张峰. 中国荷斯坦牛BOLA-DRB3外显子2的PCR-SSCP多态性分析[J]. 华北农学报, 2010, 25(5): 94-98. doi: 10.7668/hbnxb.2010.05.020.

WANG Xueqing, WANG Kun, WANG Peiyu, LI Geng, SUN Dongxiao, WU ZhanjunZHANG Feng1. PCR-SSCP Analysis of Genetic Polymorphisms of BoLA-DRB3 Exon 2 Gene in Chinese Holstein[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 94-98. doi: 10.7668/hbnxb.2010.05.020.

http://www.hbnxb.net/CN/Y2010/V25/I5/94

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中国荷斯坦牛BOLA-DRB3外显子2的PCR-SSCP多态性分析

PCR-SSCP Analysis of Genetic Polymorphisms of BoLA-DRB3 Exon 2 Gene in Chinese Holstein

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中国荷斯坦牛BOLA-DRB3外显子2的PCR-SSCP多态性分析

PCR-SSCP Analysis of Genetic Polymorphisms of BoLA-DRB3 Exon 2 Gene in Chinese Holstein

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