摘要： 以玉米大斑病菌野生型菌株01-23为供试菌株,观测不同浓度水杨酸处理对病菌分生孢子萌发、菌落生长速度及形态、菌丝发育等方面的影响。结果表明,30μg/mL水杨酸处理对病菌分生孢子萌发有显著的促进作用,处理后24h孢子萌发率是对照的1.53倍。不同浓度水杨酸处理对病菌菌落生长速度也有明显促进作用:15μg/mL水杨酸处理,第8天菌落的生长速度提高了86%;30μg/mL水杨酸处理,第8天菌落的生长速度提高了61%;60μg/mL水杨酸处理,第10天菌落生长速度提高了59%。不同浓度水杨酸处理对菌丝发育也有显著影响:水杨酸浓度为15μg/mL时,菌丝发育没有明显变化;水杨酸浓度为30μg/mL和60μg/mL时,菌丝直径及菌丝形态也无明显变化,但部分菌丝中致密的内含物减少,出现了较分散的空泡状结构,且畸形菌丝的数目增多。水杨酸处理对分生孢子的形态、产生时间及菌落颜色均无明显的影响。

关键词: 玉米大斑病菌, 水杨酸, 生长发育

Abstract: Isolate 01-23 from Setosphaeria turcica was chose as the experimental material to research the effects of salicylic acid(SA)on the growth and development,which including the germination rate of conidia,the growth rate of colony,colony morphology,and the mycelium morphology and so on.The results showed that SA supplement at 30 μg/mL in the PDA medium significantly accelerated the conidial germination of the isolate and increased by 1.53 times.There was obvious promotion to the growth rate of colony when the concentration of SA was added to PDA medium.The growth rate increased by 86% at the eighth day when the concentration of SA was 15 μg/mL.The growth rate increased by 61% at the eighth day when the concentration of SA was 30 μg/mL.The growth rate increased by 59% at the tenth day when the concentration of SA was 60 μg/mL.There was also evident effect on the development of mycelium under the treatment of SA.It was not be found that there was distinct influence on the development of mycelium when the concentration of SA was 15 μg/mL.There was also no change in the diameter and morphology of the mycelium when the concentration of SA were 30 μg/mL and 60 μg/mL,while reducing compacted contents,scattered vacuoles,and abnormal mycelium were found in some cell.There was no evident effect on the morphology,the arising time of conidia,and the color of the colony.

Key words: Setosphaeria turcic, Salicylic acid, Growth and development

中图分类号: 

• S435.13