甜高粱重要种质材料的SRAP指纹分析

doi:10.7668/hbnxb.2010.02.018

摘要/Abstract

摘要： 使用27个SRAP引物组合,对47个重要的甜高粱种质材料进行了遗传相关性和遗传多样性的分析。结果产生了可以明确区分所有参试的甜高粱基因型的SRAP指纹图谱,聚类分析将它们分为在糖产量性状及其他农艺、形态性状上具有明显差异的3类,其中第I类为具有高Brix和高糖产量的基因型,第Ⅲ类中的大多数基因型则与此相反。所建立的47个甜高粱基因型的SRAP指纹图谱可用于这些基因型的鉴定,同时,所得到遗传相关性与遗传多样性信息为进一步的遗传研究和杂交亲本的选择提供了参考依据。

关键词: 甜高粱, 基因型, SRAP, 遗传相关, 遗传多样性

Abstract: This study used 27 SRAP primer pairs to analyze genetic relationships and genetic diversity among 47 elite sweet sorghum gemplasm.The results showed that the studied genotypes could be definitively distinguished by the SRAP fingerprinting patterns generated from these primer pairs.Furthermore,cluster analysis based on SRAP data classfied the studied genotypes into 3 major groups,and this result largely consistent with the phylogenetic relationships of the studied genotypes based on sugar yield traits and morphologic traits.In these 3 groups,all of genotypes belonged to group I had a high sugar yield and Brix while the reverse was true for most genotypes from group III.The fingerprinting patters produced by this study could be applied for identification of the studied genotypes,and the resulted information on genetic relationships and genetic diversity among 47 elite sweet sorghum gemplasm provided a good base for choosing cross parents in genetic researches and developing new varieties.

Key words: Sweet sorghum, Genotype, SRAP, Genetic relationships, Genetic diversity

中图分类号:

S514.024

高建明, 罗峰, 裴忠有, 陈秋玲, 魏进招, 李子芳, 桂枝, 孙守钧. 甜高粱重要种质材料的SRAP指纹分析[J]. 华北农学报, 2010, 25(2): 93-98. doi: 10.7668/hbnxb.2010.02.018.

GAO Jian-ming, LUO Feng, PEI Zhong-you, CHEN Qiu-ling, WEI Jin-zhao, LI Zi-fang, GUI Zhi, SUN Shou-jun. SRAP Fingerprinting of 47 Elite Sweet Sorghum Germplasm[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 93-98. doi: 10.7668/hbnxb.2010.02.018.

http://www.hbnxb.net/CN/Y2010/V25/I2/93

参考文献

[1] 贾虎森,许亦农.生物柴油利用概率及其在中国的发展思路[J].植物生态学报,2006,30(2):221-230.
[2] 李军,吴平治,李美茹,等.能源植物的研究进展及其发展趋势[J].自然杂志,2007,19(1):21-25.
[3] Becelaere G V,Edward L L,Paterson A H,et al.DNA marker-based genetic similarity estimates in cotton[J].Crop Sci,2005,45:2281-2287.
[4] Menz M A,Klein R R,Unruh N C,et al.Genetic diversity of public inbreds of sorghum determined by mapped AFLP and SSR markers[J].Crop Sci,2004,44:1236-1244.
[5] Kamala V,Brame P J,Sivaramakrishnan S,et al.Genetic and phenotypic diversity in downy-mildew-resistant sorghum(Sorghum bicolor(L.)Moench)germplasm[J].Genet Resour Crop Evol,2006,53:1243-1253.
[6] Nemera G,Maryke T,Abuschagne L,et al.Genetic diversity analysis in sorghum germplasm as estimated by AFLP,SSR and morpho-agronomical markers[J].Biodiversity and Conservation,2006,15:3251-3265.
[7] Folkertsma R,Frederick H,Rattunde W,et al.The pattern of genetic diversity of Guinea-race Sorghum bicolor(L.)Moench landraces as revealed with SSR markers[J].Theor Appl Genet,2005,111:399-409.
[8] Uptmoor R,Wenzel W,Friedt W,et al.Comparative analysis on the genetic relatedness of Sorghum bicolor accessions from Southern Africa by RAPDs,AFLPs and SSRs[J].Theor Appl Genet,2003,106:1316-1325.
[9] Wu Y Q,Huang Yinghua,Tauer C G,et al.Genetic diversity of sorghum accessions resistant to greenbugs as assessed with AFLP markers[J].Genome,2006,49:143-149.
[10] Nkongolo K K,Nsapato L.Genetic diversity in Sorghum bicolor(L.)Moench accessions from different ecogeographical regions in Malawi assessed with RAPDs[J].Genetic Resources and Crop Evolution,2003,50:149-156.
[11] Deu M,Rattunde F,Chantereau J.A global view of genetic diversity in cultivated sorghums using a core collection[J].Genome,2005,49:168-180.
[12] Ritter K B,Lynne M C,Godwin I D,et al.An assessment of the genetic relationship between sweet and grain sorghums,within Sorghum bicolor ssp.bicolor(L.)Moench,using AFLP markers[J].Euphytica,2007,157:161-176.
[13] Ali M L,Rajewski J F,Baenziger P S,et al.Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplasm by SSR markers[J].Mol Breeding,2008,21:497-509.
[14] Li G,Quiros C F.Sequence-related amplified polymorphism(SRAP),a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103:455-461.
[15] Budak H R C,Shearman I,Parmaksiz R E,et al.Molecular characterization of Buffalo grass germplasm using sequence-related amplified polymorphism markers[J].Theor Appl Genet,2004,108:328-334.
[16] Vandemark G J,Ariss J J G A,Bauchan R C,et al.Estimating genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphisms[J].Euphytica,2006,152:9-16.
[17] Sun Z D,Wang Z N,Tu J X,et al.An ultradense genetic recombination map for Brassica napus,consisting of 13551 SRAP markers[J].Theor Appl Genet,2007,114:1305-1317.
[18] Doyle J F,Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Focus,1990,12:13-15.
[19] Smith J S C,Chin E C L,Shu H,et al.An evaluation of the utility of SSR loci as molecular markers in maize(Zea mays L.):comparisons with data from RFLPs and pedigree[J].Theor Appl Genet,1997,95:163-173.
[20] Ferriol M,Pic B,Nuez F.Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP marker[J].Theor Appl Genet,2003,107:271-282.

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