华北农学报

所属专题: 玉米 生物技术

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一个玉米氯离子通道基因(clc)的电子克隆及生物信息学分析

张桂荣   

  1. 河北科技师范学院图书馆 河北 秦皇岛 066004
  • 收稿日期:2009-10-25 出版日期:2009-12-31
  • 作者简介:张桂荣(1972-), 在职硕士, 馆员, 主要从事图书、生物信息学数据库技术服务工作.
  • 基金资助:
    河北省教育厅项目(2008499)

In Silico Cloning of Chloride Channel Gene(clc) from Zea mays and Bioinformatic Analysis

ZHANG Gui-rong   

  1. Library Hebei Normal University of Science and Technology, Qinhuangdao 066004, China
  • Received:2009-10-25 Published:2009-12-31

摘要: 氯离子通道植物生理代谢中起着重要的角色。通过电子克隆的方法获得玉米氯离子通道的cDNA全长序列。发现该基因包括5个外显子和4个内含子, 并对其表达产物进行生物信息学分析, 其表达产物与拟南芥ATCLC-A, ATCLC-B具有较高的相似性。

关键词: 玉米, clc, 氯离子通道, 电子克隆, 生物信息学

Abstract: Chloride Channel play an inportant role in the plant physiology. The cDNA of Chloride Channel gene (clc) from Zea Mays was isolated through In Silico Cloning. The results of sequence Analysis show that it includes 5 exons and 4 introns. Express product of clc gene was analysis by bioinformatics method, this hypothesis protein shows high similarity with Arabidopsis thaliana ATCLC-A, ATCLC-B.

Key words: Zea mars, clc, Chloride channel, In silico Cloning, Bioinformatic

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引用本文

ZHANG Gui-rong. In Silico Cloning of Chloride Channel Gene(clc) from Zea mays and Bioinformatic Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S2.006.

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