华北农学报 ›› 2009, Vol. 24 ›› Issue (5): 135-139. doi: 10.7668/hbnxb.2009.05.028

所属专题: 小麦

• 论文 • 上一篇    下一篇

镉对小麦种子萌发、幼苗生长及抗氧化酶活性的影响

何俊瑜, 任艳芳, 任明见, 王阳阳   

  1. 贵州大学农学院, 贵州贵阳 550025
  • 收稿日期:2009-05-04 出版日期:2009-10-28
  • 作者简介:何俊瑜(1975-),男,山西河曲人,副教授,博士,主要从事环境生理生态方面的研究。
  • 基金资助:
    贵州省科技厅成果推广计划(200880);贵州省农业厅动植物育种专项项目(2007003);贵州省自然科学基金(20072058)

Effect of Cadmium Stress on Seed Germination,Seedling Growthand the Activities of Antioxidant Enzymes of Wheat

HE Jun-yu, REN Yan-fang, REN Ming-jian, WANG Yang-yang   

  1. College of Agronomy, Guizhou University, Guiyang 550025, China
  • Received:2009-05-04 Published:2009-10-28

摘要: 为了防止重金属毒害的发生和在环境监测中对重金属的评价,以小麦为试验对象,采用室内培养方法,研究了不同浓度镉胁迫对小麦种子萌发和幼苗生长、丙二醛及抗氧化酶系统的影响,结果表明,较低浓度镉胁迫对种子发芽率、发芽势、芽生长的抑制效应较小。随着镉胁迫浓度的提高,发芽指数、活力指数及根生长明显受抑制,且镉胁迫浓度越大,抑制作用越大。随着镉浓度的增加,幼苗根、芽中的MDA含量、G-POD明显增加,SOD活性呈现先增加后降低的趋势,其中根的SOD活性在1000μmol/L Cd胁迫下略低于对照,而CAT、APX活性总体呈下降趋势,且浓度越高下降幅度越大,表明遭受了氧化胁迫和膜脂过氧化损伤,这可能是镉胁迫下小麦幼苗根生长受阻的重要原因。

关键词: 镉, 小麦, 萌发, 幼苗生长, 抗氧化酶

Abstract: Cadmium (Cd)was a highly toxic and persistent environmental poison,its increasing level in soil environment exerted a wild range of adverse effects on plants and even the healthy of human beings by food chains.In the study,the effects of Cd at different concentrations on wheat seeds germination,seedlings growth and the activities of antioxidant enzymes were studied.The results showed the germination percentage,germination potential and shoot growth were not significantly inhibited by cadmium,especially at low Cd concentrations.However,germination index,vigor index,root growth were restrained evidently with the increase of Cd stress.The content of MDA and activities of G-POD in root and shoot increased significantly with the increasing of Cd concentration.The activities of SOD first increased and then decreased as the Cd concentration increased and it was slight lower than that of control in root under 1000μmol/ L Cd stress.However,the activities of CAT and APX were decreased with concentration-dependent manner,ie,the higher concentration of cadmium,the greater changes the enzyme activities,suggesting oxidative damage and membrane lipid peroxidation occurred in seedlings,and it might be the important reason for the growth inhibition of roots subjected to cadmium stress.

Key words: Cd stress, Wheat, Germination, Seedling growth, Antioxidant enzymes

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引用本文

何俊瑜, 任艳芳, 任明见, 王阳阳. 镉对小麦种子萌发、幼苗生长及抗氧化酶活性的影响[J]. 华北农学报, 2009, 24(5): 135-139. doi: 10.7668/hbnxb.2009.05.028.

HE Jun-yu, REN Yan-fang, REN Ming-jian, WANG Yang-yang. Effect of Cadmium Stress on Seed Germination,Seedling Growthand the Activities of Antioxidant Enzymes of Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 135-139. doi: 10.7668/hbnxb.2009.05.028.

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