摘要： 以普通栽培型番茄辽园多丽为试验材料,研究了花期施用PCPA对发育过程中的番茄果实糖含量变化、蔗糖代谢关键酶活性及可溶性酸性转化酶基因表达的影响.结果表明,随着番茄果实的发育,可溶性酸性转化酶的活性和酶基因的表达均增强,果糖和葡萄糖的含量也呈递增的趋势,成熟时含量最高.PCPA处理在果实发育的成熟期提高了酸性转化酶的活性,促进了可溶性酸性转化酶基因的表达,增加了果糖和葡萄糖的含量.

关键词: 番茄, 外源生长素, 蔗糖代谢, 酸性转化酶, 基因表达

Abstract: Sugar contents,activities of related enzymes and gene expression of soluble acid invertase during tomato fruit development by PCPA treatment were studied. The results exhibited that activity of acid invertase and gene expres2 sion of soluble acid invertase enhanced duringfruit development,while the contents of fructose and glucose increased. The activity of acid invertase was improved and gene expression of soluble acid invertase was accelerated during mature period of fruit after PCPA treatment so that the contents of fructose and glucose were increased.

Key words: Lycopersicon esculentum Mill., Exogenous auxin, Sucrose metabolism, Acid invertase, Gene expression

中图分类号: 

• S641.01