摘要： 通过4x-2x杂交测验,从Solanum phureja后代中筛选出9份诱导双单倍体频率和花粉育性高的授粉者,它们的平均诱导率为33株双单倍体/100个浆果,并具有显性纯合的胚斑标记基因(BB)和紫色胚轴标记基因(PP),可用来有效识别双单倍体。不同的四倍体母本产生双单倍体的频率不同,中薯2号产生频率最高,100个果中可产生34株双单倍体,母本基因型对双单倍体的产生有很大的影响。

关键词: 马铃薯, 双单倍体, 孤雌生殖, 标记基因

Abstract: Through 4x-2x cross,9 lines from Solatium phureja were selected as pollinators. These pollinators had several advantages over the controls (IVP35 and IVP48) , including high frequency of dihaploid induction and pollen fertility. Their average frequency of induction was 33.0 dihap-loids/100 fruits. They also possessed a dominant embryo spot marker (BB) and a dominant seed-ing marker (PP) for purple hypocotyl. Both markers are homozygous and can be effcetively used to inentify dihaploids. There were differences in the dihaploid frequency between the maternal parents. Zhongshu 2 had the highest frequency (34.0 dihaploid/100 fruits) among three varicties. But the effects of environments on pseudogamous dihapoid production can never be neglected.

Key words: Potato, Dihaploid, Parthenogenesis, Marker gene

中图分类号: 

• S532