摘要： 利用穗粒数和穗长差异较大的2个粳稻品种日本晴与大穗型粳稻种质B0801组配群体,对水稻穗粒数、穗长和着粒密度等穗部性状进行数量基因位点检测,共检测到11个QTL,分布于第1,2,5,7以及9号染色体上,QTL的贡献率范围为0.21%～68.20%。其中,4个控制穗粒数的QTL分别位于第1,2,7,9号染色体,其贡献率为0.21%～25.06%。第9号染色体RM1328～RM3533标记区间的控制穗粒数性状的qGNP9-1位点,为主效QTL;控制穗长的QTL 4个,4个QTL分别位于第1,2,5,9号染色体。其贡献率范围为0.28%～61.19%,其中qPL9-1的LOD值为31.88,对表型变异的贡献率为68.19%,为主效QTL。控制着粒密度的QTL 3个,位于第1,2,9号染色体,其贡献率为0.43%～6.91%。qSD1-1增效等位基因来自大穗亲本,基因作用方式为加性。
关键词:
水稻,
穗长,
着粒密度,
穗粒数,
QTL
Abstract: A F2 population from Nipponhare and 80801 ( a bid-ear variety with more spikelets and gains) was used in the study，and 11 QTLs associated with grain number， spike length and grain density were detected，which distributed on the chromosome 1，2，5，7，9 respectively． The LOD value was 2.03-31.88，and the contribution rate of phenotype ranged from 0.21% to 68.20%．4 QTLs were associated with grain number，distributing on the chromosome 1，2，7 and 9，respectively，with the LOD value 2.11-4.00 and the genotype contribution rate 0.21%-25.06%． 4 QTLs about spikelet length were mapped on chromosome 1，2，5，9 the LOD value being 2.03-31.88and the genotype contribution 0.28%-61.19%． Among them，qPL9-1 had the LOD value of 31.88 and contribution rate of 68.19%，which should be a major QTL． Besides，3 QTLs about grain density were located on chromosome 1，2，9 the LOD value being 2.15-2.64 and the phenotype contribution rate being 0.43%-6.91.
Key words:
Rice,
Spike length,
Grain density,
Grain number,
QTL
中图分类号:
潘英华, 余建平, 张强, 熊海燕, 朱汝财, 李自超. 水稻穗粒数相关基因的初步定位[J]. 华北农学报, 2013, 28(3): 19-27. doi: 10.3969/j.issn.1000-7091.2013.03.004.
Pan Yinghua, Yu Jianping, Zhang Qiang, Xiong Haiyan, Zhu Rucai, Li Zichao. Mapping QTL Associated with Grain Number of Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 19-27. doi: 10.3969/j.issn.1000-7091.2013.03.004.