华北农学报 ›› 2012, Vol. 27 ›› Issue (6): 67-71. doi: 10.3969/j.issn.1000-7091.2012.06.015

所属专题: 水稻

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华南籼型杂交稻常用恢复系遗传差异分析

阎勇, 黄大辉, 秦钢, 梁海福, 屈秋明, 粟学俊   

  1. 广西水稻遗传改良重点开放实验室, 广西农科院水稻研究所, 国家水稻改良中心南宁分中心, 广西南宁 530007
  • 收稿日期:2012-06-02 出版日期:2012-12-28
  • 通讯作者: 栗学俊(1966-),男,广西南宁人,研究员,硕士,主要从事杂交水稻育种研究。
  • 作者简介:阎勇(1979-),男,山西忻州人,助理研究员,硕士,主要从事杂交水稻育种研究。
  • 基金资助:
    广西自然科学基金项目(2010GXNSFB013033);广西农科院基本业务专项(201010基)

Genetic Diversity of Main Restorer Lines of Hybrid Rice in South China

YAN Yong, HUANG Da-hui, QIN Gang, LIANG Hai-fu, QU Qiu-ming, SU Xue-jun   

  1. Key Laboratory of Rice Genetic Improvement of Guangxi, Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning Subcenter of the National Center for Rice Improvement, Nanning 530007, China
  • Received:2012-06-02 Published:2012-12-28

摘要: 利用分布于水稻12条染色体上已筛选的24个微卫星标记,对华南地区常用的30份杂交稻恢复系进行遗传差异分析。所有引物检测到的等位基因是70个,平均每对引物可检测到2.5个等位基因;有效等位基因数介于1.069~4.000之间,平均为2.051;多态信息含量(PIC)变动范围为0.064~0.750,平均为0.456。聚类分析表明,30个水稻恢复系间遗传相似系数在0.58~0.97之间,有4份材料桂55、9518、测315和5058与其他材料间的遗传差异较大,可在强优势组合的培育中加以利用。

关键词: 水稻, 恢复系, 遗传差异, 分子标记

Abstract: Twenty-four SSR on the 12 chromosomes of rice ( Oryza sativaL. ) were selected to assess the genetic variation among 30 main restorer lines of hybrid rice in South of China. A total of 70 alleles were detected,and per primer pair detected 2. 5 alleles on the average. The number of effective alleles ranged from 1. 069 to 4. 000,on an average of 2. 051. The polymorphism information content ( PIC) ranged from 0. 064 to 0. 750,on an average of 0. 456. The result of cluster analysis showed that the genetic similarity among 30 rice restorer lines ranged from 0. 58 to 0. 97. Four restorer lines Gui55, 9518,Ce315 and 5058 showed greater genetic diversity in comparison with the other restorer lines. The above 4 restorer lines could be used to breed hybrid rice with strong superiority.

Key words: Rice ( Oryza sativaL. ) , Restorer lines, Genetic diversity, SSR marker

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引用本文

阎勇, 黄大辉, 秦钢, 梁海福, 屈秋明, 粟学俊. 华南籼型杂交稻常用恢复系遗传差异分析[J]. 华北农学报, 2012, 27(6): 67-71. doi: 10.3969/j.issn.1000-7091.2012.06.015.

YAN Yong, HUANG Da-hui, QIN Gang, LIANG Hai-fu, QU Qiu-ming, SU Xue-jun. Genetic Diversity of Main Restorer Lines of Hybrid Rice in South China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 67-71. doi: 10.3969/j.issn.1000-7091.2012.06.015.

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