摘要： 为探究辣椒不育材料FS1030A的败育发生时期及方式,利用透射电镜观察技术对FS1030A与相应保持系FS1030B不同发育时期的花药进行了细胞形态学观察。结果表明,不育系FS1030A败育发生在四分体形成前。小孢子母细胞减数分裂时期,绒毡层细胞结构发生了2种异常变化:部分绒毡层细胞高度液泡化,径向过度伸长,后期生长为多层细胞,导致药室狭小,严重挤压小孢子母细胞致使其发育畸形,进而退化解体,不能形成正常的四分体小孢子;部分绒毡层细胞过早解体,解体的绒毡层细胞与小孢子母细胞残迹在药室中形成一条染色较深的带。绒毡层异化,中层不解体,不能为小孢子母细胞的进一步发育提供营养,从而造成花粉母细胞变形、降解,最终败育。
关键词:
辣椒,
细胞质雄性不育,
透射电镜观察技术,
绒毡层
Abstract: Transmission Electronic Microscopy(TEM) technique was used to observe the abortive stages and ways of FS1030A,a cytoplasmic male sterile line(CMS) in Capsicum annum L.and its maintainer line FS1030B.The results showed that the abortion occurred before tetrads came into being,and two kinds of main structural changes in the tapetum were observed in CMS FS1030A.First during the meiosis of microspore mother cells(MMCs),some of the tapetal cells were enlarged,elongated and developed into multilayer cells.They squeezed the MMCs excessively which narrow the powder chamber much.The MMCs degenerated,vanished and failed to develop into tetrads,eventually leading to microspore abortion.Second some of the premature tapetal cell layer degenerated,and then the remnants of the collapsed tapetum and crushed MMCs formed a visible dense belt in the anther locule.The MMCs presented irregular shapes,and eventually degenerated due to the lack of nutrition.
Key words:
Capsicum annum,
L Cytoplasmic male sterility(CMS),
TEM Tapetum
中图分类号:
张玲玲, 张晓芬, 陈斌, 孙艳, 韩华丽, 耿三省. 辣椒细胞质雄性不育系FS1030A小孢子发生的细胞形态学观察[J]. 华北农学报, 2012, 27(5): 122-126. doi: 10.3969/j.issn.1000-7091.2012.05.024.
ZHANG Ling-ling, ZHANG Xiao-fen, CHEN Bin, SUN Yan, HAN Hua-li, GENG San-sheng. Cytologic Observations on Microspore Development of FS1030A,a Cytoplasmic Male Sterile Line in Capsicum annum L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 122-126. doi: 10.3969/j.issn.1000-7091.2012.05.024.