华北农学报 ›› 2012, Vol. 27 ›› Issue (2): 97-99. doi: 10.3969/j.issn.1000-7091.2012.02.019

所属专题: 玉米

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利用种子性状QTL定位高油玉米淀粉含量QTL

李学慧1, 申顺先1, 李玉玲2, 曹雯梅1, 杜红1   

  1. 1. 河南农业职业学院, 河南中牟 451450;
    2. 河南农业大学农学院, 河南郑州 450002
  • 收稿日期:2010-12-01 出版日期:2012-04-28
  • 作者简介:李学慧(1979-), 女, 河南驻马店人, 讲师, 硕士, 主要从事作物遗传育种教学与研究工作。
  • 基金资助:
    河南省科技攻关项目(0424020025)

QTL Analysis of Starch Content in High-oil Maize Using Seed Trait QTL

LI Xue-hui1, SHEN Shun-xian1, LI Yu-ling2, CAO Wen-mei1, DU Hong1   

  1. 1. Henan Vocational College Agriculture, Zhongmu 451450, China;
    2. Agronomy College of Henan Agricultural University, Zhengzhou 450002, China
  • Received:2010-12-01 Published:2012-04-28

摘要: 以普通玉米自交系8984与高油玉米自交系GY220为亲本组配得到284个F2:3家系群体,利用185个SSR标记构建玉米遗传连锁图谱。通过包含母体效应的种子性状QTL作图方法对玉米籽粒淀粉含量进行QTL定位和效应分析,共检测到7个与淀粉含量相关的QTL,分别位于第5,8,10染色体上,除qSTA8-3的遗传作用方式表现为加性外,其余QTL作用方式为部分显性。单个QTL贡献率为5.87%~10.93%,累计贡献率为53.37%。所有QTL的增效基因均来自普通玉米亲本8984。淀粉含量QTL qSTA5-2贡献率较大,可以作为进一步精细定位的主要目标QTL。

关键词: 高油玉米, 淀粉含量, 种子性状QTL, QTL定位

Abstract: F2:3populations consisted of 284 plants were developed from the cross between a normal maize inbred 8984 and a high-oil maize inbred GY220,and a genetic linkage map including 185 SSR markers constructed?by (8984/GY220) F2population. The quantitative trait loci (QTL) of starch content were identified and their?effects were evaluated using SeedTraitQTL containing maternal effect. A total of seven QTL were detected in the F2:3?population,located on chromosome 5,8 and 10 respectively. Six QTLs showed partially dominant effects but qSTA83 showed additive. Contribution of single QTL to phenotypic variation varied from 5. 87% to 10. 93%,and total contributions of all QTLs were 53. 38%. The allele of all QTLs were contributed by the normal maize parent 8984. The?QTL qSTA5-2 with higher contribution could be used as the main objective QTL in fine mapping.

Key words: High-oil maize, Starch content, Seed Trait QTL, QTL analysis

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引用本文

李学慧, 申顺先, 李玉玲, 曹雯梅, 杜红. 利用种子性状QTL定位高油玉米淀粉含量QTL[J]. 华北农学报, 2012, 27(2): 97-99. doi: 10.3969/j.issn.1000-7091.2012.02.019.

LI Xue-hui, SHEN Shun-xian, LI Yu-ling, CAO Wen-mei, DU Hong. QTL Analysis of Starch Content in High-oil Maize Using Seed Trait QTL[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 97-99. doi: 10.3969/j.issn.1000-7091.2012.02.019.

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