不同施氮水平下水稻株高与剑叶性状QTL比较分析

doi:10.7668/hbnxb.201751677

摘要/Abstract

摘要： 为了揭示不同氮素环境下株高和叶片性状的分子遗传基础，鉴定新的环境钝感的主效QTL。以丰锦和中优早8杂交衍生的籼粳交重组自交系（RILs）群体为试验材料，在不同氮素水平下对株高和叶片性状进行QTL分析。不同施氮水平双亲的株高和叶片性状存在明显变化，中优早8对氮素响应程度明显高于丰锦，不同施氮水平株高和叶片性状具有较高的遗传力。共检测到38个相关性状的QTL，分布于10条染色体上的17个染色体区域，仅9个QTL在不同供氮水平下稳定表达，氮素水平差值性状相关QTL主要分布在第7，8，11号染色体上。检测到2个新的环境钝感的主效QTL簇qPHLW7和qFLWA10，位于第7染色体的多效性QTL簇qPHLW7，具有明显的调控株高、叶长和叶宽的功能；位于第10染色体上的主效QTL簇qFLWA10参与调控剑叶宽和剑叶面积，具有明显的延伸叶片宽度和面积的功能。共检测到23对互作QTL，上位性互作是调控株高和叶片性状的重要遗传组成，且主效QTL参与上位性互作效应。检测到9个环境钝感表达的主效QTL，其中主效QTL簇qPHLW7和qFLWA10是调控叶片和株高性状的2个关键染色体区域，相关研究为后续探究奠定了基础。

关键词: 水稻, 氮素水平, 株高, 剑叶性状, QTL定位

Abstract: To reveal the molecular genetic basis of plant height and leaf traits of rice in different nitrogen environments and identify the novel environmental insensitive major QTL cluster, a recombinant inbred lines(RILs) derived from the cross between Toyonishiki(Japonica) and Zhongyouzao 8(Indiaca) was used to identify QTLs for plant height and leaf traits under two different nitrogen levels. The plant height and leaf traits of the parents changed obviously under different nitrogen levels, and the change of Zhongyouzao 8 was significantly higher than that of Toyonishiki. The plant height and leaf traits had higher heritability under different nitrogen levels. A total of thirty-eight QTLs affecting the plant height and leaf traits were identified, which distributed in 17 regions of 10 chromosomes. Among the QTLs detected, nine could be stably expressed in different nitrogen levels, which mainly distributed on chromosomes 7, 8 and 11. Two new stable major QTL clusters qPHLW7 and qFLWA10 were detected.qPHLW7 was located on chromosome 7 and had the function of regulating plant height, leaf length and leaf width. qFLWA10 was located on chromosome 10, which involved in the regulation of flag leaf width and flag leaf area and had obvious function of extending leaf width and area. In addition, a total of 23 epistatic QTLs were detected, and epistatic interaction was one of the most important genetic component to regulate the plant height and leaf traits. Nine stable expressive major QTL were detected, in which the QTL cluster qPHLW7 and qFLWA10 were two key chromosome regions for leaf and plant height traits, and then it will provide a foundation for further study.

Key words: Rice, Nitrogen level, Plant height, Flag leaf, QTL mapping

中图分类号:

S511.03

姚晓云, 范淑秀, 邹国兴, 吴延寿, 陈春莲, 熊运华, 刘进, 王嘉宇, 徐正进. 不同施氮水平下水稻株高与剑叶性状QTL比较分析[J]. 华北农学报, 2019, 34(6): 54-62. doi: 10.7668/hbnxb.201751677.

YAO Xiaoyun, FAN Shuxiu, ZOU Guoxing, WU Yanshou, CHEN Chunlian, XIONG Yunhua, LIU Jin, WANG Jiayu, XU Zhengjin. QTLs Analysis for Plant Height and Leaf Traits of Rice under Two Nitrogen Levels[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 54-62. doi: 10.7668/hbnxb.201751677.

http://www.hbnxb.net/CN/Y2019/V34/I6/54

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