摘要： 扩增了自2006-2009年间从河南省不同地区分离的9株PRRSV株NSP2和GP5编码基因,并进行了序列测定和分析。结果显示,9株分离株NSP2全长2 850～2 937 bp,编码950～979个氨基酸,GP5基因全长603 bp,编码200个氨基酸,与CH-1a株比对,其中有7株分离株的NSP2基因均出现30个氨基酸的缺失。对9株分离株NSP2和GP5基因进行核苷酸序列分析并绘制进化树,结果表明,9株河南分离株都属于美洲型毒株,对9株PRRSV分离株NSP2和GP5基因同时进行序列分析,比较其变异结果是否一致,为进一步探寻PRRSV流行株遗传变异规律奠定了基础。
关键词:
PRRSV,
NSP2基因,
ORF5基因,
克隆变异分析
Abstract: Amplifying the NSP2 and GP5 genes sequences of nine strains PRRSV isolated from different areas?of Henan between 2006 and 2009，these genes of NSP2 exhibited different sizes in length ranging from 2 850 bp to?2 937 bp and coded 950 －979 aa，and the length of GP5 were 603 bp and coded 200 aa． Nucleotide and deduced amino acid sequences analysis revealed that 7 out of 9 Nsp2 sequences contained the same 30 aa deletion and the results indicated that 9 strains were American． In order to compare the consistency between Nsp2 and GP5 variations, the Nsp2 and GP5 genes sequence of nine strains PRRSV genome isolated from Henan were analyzed，then exploring?the genetic variation characteristics of epidemic PRRSV strains．
Key words:
Porcine reproductive and respiratory syndrome virus,
NSP2 gene,
ORF5 gene,
Variation sequence
中图分类号:
卢晓艳, 周永辉, 李伟娟, 张志远, 刘肖萍, 段二珍, 夏平安, 崔保安. 猪繁殖与呼吸综合征病毒河南分离株NSP2和GP5基因遗传变异分析[J]. 华北农学报, 2012, 27(2): 100-104. doi: 10.3969/j.issn.1000-7091.2012.02.020.
LU Xiao-yan, ZHOU Yong-hui, LI Wei-juan, ZHANG Zhi-yuan, LIU Xiao-ping, DUAN Er-zhen, XIA Ping-an, CUI Bao-an. Analysis of NSP2 and GP5 Gene Variation of Porcine Reproductive and Respiratory Virus Strain Isolated in Henan Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 100-104. doi: 10.3969/j.issn.1000-7091.2012.02.020.