摘要： 研究山药多糖对PRRSV灭活苗免疫抗体和猪外周血T细胞亚群的影响。选择35日龄断奶仔猪16只,随机分为4组,将山药多糖分别以17.1mg/kg和8.55mg/kg两个剂量和猪PRRSV灭活苗同时注射,于免疫后7,14,24,34,44,54,69,79d采血,ELISA方法检测猪PRRSV抗体水平,流式细胞术检测猪外周血中T淋巴细胞亚群的变化。结果表明,山药多糖可显著提高PRRSV灭活苗免疫猪外周血CD3+和CD8+细胞数量,在免疫34d后,两个剂量的山药多糖均可显著提高PRRSV灭活苗免疫抗体水平。山药多糖可以作为免疫增强剂与PRRSV灭活苗联合使用。
关键词:
山药多糖,
PRRSV灭活苗,
抗体,
T淋巴细胞亚群
Abstract: The research is to study the effects of Chinese yam polysaccharide(CYPS)on antibody and T subpopulations in piglets pheripheral blood in the immune response to PRRSV killed virus vaccine.16 35-day-old piglets were randomly assigned into equal four groups and three groups were vaccinated with PRRSV killed virus vaccine.At the same time,the piglets in groups CYPSH and CYPSL were intramuscularly injected with CYPS at 17.1 or 8.55 mg/kg dosages,group C(control group),with sterile saline,once a day for three successive days,group F(fake group)only injected with saline.At 7,14,24,34,44,54,69,79 day after vaccination,pheripheral blood were sampled for detection of serum antibody titer to PRRSV by ELISA and T lymphocytes subpopulations by cytofluorometry.Results showed that CYPS can boost the proportion of CD3+ and CD8+ lymphocytes in piglets pheripheral blood after immunization with PRRSV killed virus vaccine,and enhanced antibody titer to PRRSV after 34 days.CYPS is a potential immunostimulatory for PRRSV killed virus vaccine.
Key words:
CYPS,
PRRSV killed virus vaccine,
Antibody,
T lymphocyte subpopulations
中图分类号:
张红英, 王学兵, 崔保安, 赵现敏, 陈红英, 金钺. 山药多糖对PRRSV灭活苗免疫猪抗体和T细胞亚群的影响[J]. 华北农学报, 2010, 25(2): 236-238. doi: 10.7668/hbnxb.2010.02.048.
ZHANG Hong-ying, WANG Xue-bing, CUI Bao-an, ZHAO Xian-min, CHEN Hong-ying, JIN Yue. Effects of Chinese Yam Polysaccharide on Antibody and T Lymphocytes Subpopulations in the Immune Response to PRRSV Killed Vaccine[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 236-238. doi: 10.7668/hbnxb.2010.02.048.