日本晴/R1126水稻重组自交系群体粒形性状QTL定位

doi:10.7668/hbnxb.2016.01.015

摘要/Abstract

摘要： 为阐明大穗型籼稻品系R1126稻谷籽粒性状的遗传机制,以其与粳稻日本晴构建的重组自交系F₁₀为材料,通过连续2年田间种植并测定各株系的籽粒粒长、粒宽和粒厚等表型性状,结合利用SSR和SFP等分子标记构建的遗传图谱,对控制该群体的稻谷粒形性状进行了QTL分析。2年试验共检测到10个控制粒长、粒宽和粒厚性状的QTL,其中qGL3-1、qGL3-2和qGL9这3个粒长QTL,以及qGW2和qGW5这2个粒宽QTL在2年试验中能被重复检测;而粒厚性状在2年试验中检测到5个QTL,但均只在1年试验中出现。根据连锁的分子标记信息, qGL3-2、qGW2和qGW5可能分别与已报道的主要粒形基因GS3、GW2和GW5等位;而qGL3-1和qGL9可能为新的粒长QTL,且在2年试验中具有很好的重演性和稳定性,两者的加性效应均能使粒长增加0.2 mm以上,对于改善稻谷外观品质性状具有较好的潜在应用价值。

关键词: 水稻, 粒长, 粒宽, 粒厚, QTL定位

Abstract: In order to clarify the genetic mechanism of grain shape traits for large panicle indica rice line,R1126,a set of recombinant inbred lines,which contain 185 lines,was developed by using R1126 crossed to the japonica rice line,Nipponbare.By measuring the grain shape traits include grain length (GL),grain width (GW) and grain thickness (GT) in two years field experiment,combining with the genetic map constructed with SSR and SFP molecular markers,QTL associated with grain shape traits were analyzed.Total ten QTL associated with GL,GW and GT,were detected in two years experiment.Among them,three QTL related to GL including qGL3-1, qGL3-2 and qGL9,and two QTL related to GW including qGW5 and qGW2,were be detected repeatedly in two years,respectively.However,no one QTL related to GT could be detected repeatedly.By comparing the molecular marker information, qGL3-2, qGW2 and qGW5 might be allele with the reported genes of GS3, GW2 and GW5,respectively.The two QTL associated with grain length, qGL3-1 and qGL9,which were expressed stably and repeated in two years experiment,exhibiting the additive effect of increasing the grain length of 0.2 mm,could be used for improving rice appearance quality.

Key words: Rice, Grain length, Grain width, Grain thickness, QTL mapping

中图分类号:

S511.03

叶乃忠, 曾盖, 唐伟, 肖应辉. 日本晴/R1126水稻重组自交系群体粒形性状QTL定位[J]. 华北农学报, 2016, 31(1): 90-95. doi: 10.7668/hbnxb.2016.01.015.

YE Naizhong, ZENG Gai, TANG Wei, XIAO Yinghui. Mapping of QTL Associated with Grain Shape Traits in Nipponbare/R1126 Recombinant Inbred Lines Population of Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 90-95. doi: 10.7668/hbnxb.2016.01.015.

http://www.hbnxb.net/CN/Y2016/V31/I1/90

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