高粱苗期耐盐碱性QTL定位

doi:10.7668/hbnxb.2015.03.008

摘要/Abstract

摘要： 为定位混合盐碱胁迫下调控高粱苗期性状的QTL位点,发掘耐盐碱基因位点及其紧密连锁标记。以耐盐碱和盐碱敏感组合BJ-299×Tx622B构建的F₂群体为材料,采用SSR标记技术和区间作图法对170个F_{2: 3}家系进行高粱苗期相对存活率(RL)、相对干质量(RDW)、相对鲜质量(RFW)和相对苗高(RSH)的QTL分析。结果表明,试验材料耐盐碱性状符合数量遗传的特点,共定位到3个QTL位点(qSAT-A、qSAT-D 和 qSAT-J)调控高粱苗期耐盐碱性状,分布在A、D和J染色体上,位于标记Sam27281~Sam22486、Sam11433~Sam78379和Sam62346a~Sam38392。 qSAT-A 和 qSAT-D 同时调控RL、RDW、RFW和RSH性状,表型贡献率分别为14.3%,9.7%,8.0%,10.6%和8.7%,13.5%,18.8%,13.6%; qSAT-J 位点调控RL性状,贡献率为7.3%。 qSAT-A 和 qSAT-D 为调控高粱苗期耐盐碱性状的重要位点,有望在高粱耐盐碱性状分子标记辅助育种发挥重要作用。

关键词: 高粱, 苗期, 耐盐碱, QTL定位, SSR标记

Abstract: Aimed to identify QTLs associated with seedling four traits under complex salt-alkaline stress in Sorghum and provide target loci and linked molecular markers for marker assisted selection of Sorghum salt-alkaline tolerant-related traits,an F₂ population with 170 lines derived from cross between BJ-299×Tx622B were used in this study.Quantitative trait loci (QTLs) for relative livability (RL),relative dry weight (RDW),relative fresh weight (RFW) and relative seedling height (RSH) at seedling stage were analyzed by interval mapping method.The results demonstrated that salt-alkaline tolerance at seedling stage of sorghum was a complex quantitative trait and controlled by multiple genes.Correlation coefficients between each two traits in the F_{2: 3} populations under complex salt-alkaline stress condition showed significant positive correlation.A total of 3 QTLs (qSAT-A, qSAT-D and qSAT-J) located on three major chromosome regions (Sam27281-Sam22486, Sam11433-Sam78379 and Sam62346a- Sam50778) on chromosome A,D and J,respectively.The negative effects were all from the salt-alkaline sensitive parent Tx622B. qSAT-A and qSAT-D were controlling four salt-alkaline tolerant related traits simultaneously,and explained phenotypic variation ranging from 8.0% to 18.8%.QTL qSAT-J for RL was detected with phenotypic variation explained of 7.3%.The two major QTLs (qSAT-A, qSAT-D) and three chromosome regions played crucial role in salt-alkaline tolerance of sorghum,which may have a potential in the practice of marker assisted sorghum breeding for improving salt-alkaline tolerance.

Key words: Sorghum, Seedling stage, Salt-alkaline tolerance, QTL mapping, SSR marker

中图分类号:

S514.03

唐朝臣, 高建明, 韩芸, 罗峰, 裴忠有, 孙守钧. 高粱苗期耐盐碱性QTL定位[J]. 华北农学报, 2015, 30(3): 42-47. doi: 10.7668/hbnxb.2015.03.008.

TANG Chao-chen, GAO Jian-ming, HAN Yun, LUO Feng, PEI Zhong-you, SUN Shou-jun. Identification of QTLs Associated with Complex Salt-alkaline Tolerance at the Seedling Stage in Sorghum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 42-47. doi: 10.7668/hbnxb.2015.03.008.

http://www.hbnxb.net/CN/Y2015/V30/I3/42

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