摘要： 为探析盐胁迫下燕麦种子萌发时蛋白质的差异表达及其相关生理功能分析,比较了燕麦在不同浓度氯化钠溶液处理下种子的萌发及幼苗生长能力,然后采用二维凝胶电泳结合基质辅助激光解析飞行时间质谱的蛋白质组学方法分析种子在0和1%氯化钠溶液处理种子萌发时的蛋白质组差异表达。结果表明:随着盐胁迫的加剧,燕麦种子的萌发力和成苗率降低。蛋白质组分析显示:1%氯化钠溶液处理使得燕麦种子蛋白质组二维凝胶中11个蛋白质点表达发生变化,其中10个蛋白质点表达量上调。质谱鉴定得到3个可靠蛋白,分别为:燕麦蛋白、燕麦蛋白N9和蛋白质二硫键异构酶。这些蛋白的差异表达可能与盐分胁迫下燕麦种子萌发的生理活动有关联。

关键词: 燕麦, 二维凝胶电泳, 蛋白质组, 盐胁迫

Abstract: To analysis the differences of protein expression and its related physiological function of oat seed germination under salt stress,the test compared the ability of oat seed germination and seedling growth in different concentrations of NaCl solution,and analyzed protein expression of oat seed imhibition in 0 and 1% NaCl solution by two-dimensional gel combined with matrix assisted laser analytical time of flight mass spectrometry proteomics methods.The results showed:the ability of oat seed germination and seedling growth reduced with the salt stress was heavy.Proteomic analysis found:eleven protein spots were altered under 1% NaCl solution,and ten of them protein expression quantity increased.At last,three reliable protein were got by mass spectrum identification.There are oat protein,oat protein N9 and key protein disulfide isomerase,respectively.The differences of these protein expression may be linked with the activity mechanism of oat seed germination under salt stress.

Key words: Avena sativa L., Two-dimensional gel electrophoresis, Protein, Salt stress

中图分类号: 

• S512.03