谷子分蘖相关QTL定位及紧密连锁标记开发

doi:10.7668/hbnxb.2018.06.005

华北农学报 ›› 2018, Vol. 33 ›› Issue (6): 33-41. doi: 10.7668/hbnxb.2018.06.005

所属专题： 杂粮作物；

谷子分蘖相关QTL定位及紧密连锁标记开发

杜晓芬¹, 王军¹, 李云飞², 王智兰¹, 袁国保³, 杜国华³, 韩芳⁴, 彭建祥³, 张文娜³, 蔡伟³, 袁峰¹, 崔巨多³, 郭二虎¹, 邹洪锋³, 张林义¹, 彭书忠¹

1. 山西省农业科学院谷子研究所, 山西省特色杂粮种质资源发掘与育种重点实验室, 山西长治 046011;
2. 华大农业应用研究院, 广东深圳 518083;
3. 华大小米产业股份有限公司, 广东深圳 518083;
4. 延安市农业科学研究所, 陕西延安 716000

收稿日期:2018-08-09 出版日期:2018-12-28
通讯作者: 王军(1972-),男,山西长子人,研究员,博士,主要从事谷子基因组学和谷子新品种选育研究。
作者简介:杜晓芬(1986-),女,山西高平人,助理研究员,硕士,主要从事谷子基因组学和谷子新品种选育研究。
基金资助:
山西省青年基金项目（2015021143）；山西省主要农作物种质创新与分子育种重点科技创新平台（201605D151002）；山西省农业科学院科技自主创新能力提升工程（2017ZZCX-09）；山西省“农谷”研发专项（YCX2017D2201）；山西省青年基金项目（201701D221199）；深圳作物分子设计育种工程（DRC-SZ[2011]863）；深圳市基金项目（JCYJ20150831201123287）；国家现代农业产业技术体系建设专项（CARS-06-13.5-A21）；农业基因组国家重点实验室（2011DQ782025）

Mapping of Quantitative Trait Locus and Developing of Closely Linked Markers for Tillering in Foxtail Millet

DU Xiaofen¹, WANG Jun¹, LI Yunfei², WANG Zhilan¹, YUAN Guobao³, DU Guohua³, HAN Fang⁴, PENG Jianxiang³, ZHANG Wenna³, CAI Wei³, YUAN Feng¹, CUI Juduo³, GUO Erhu¹, ZOU Hongfeng³, ZHANG Linyi¹, PENG Shuzhong¹

1. Millet Research Institute, Shanxi Academy of Agricultural Sciences, Shanxi Key Laboratory of Genetic Resources and Breeding in Minor Crops, Changzhi 046011, China;
2. BGI Institute of Applied Agriculture, Shenzhen 518083, China;
3. BGI Millet Co., Ltd, Shenzhen 518083, China;
4. Research Institute of Agriculture Sciences of Yan'an, Yan'an 716000, China

Received:2018-08-09 Published:2018-12-28

摘要/Abstract

摘要： 分蘖是与农作物产量相关的重要农艺性状之一，分蘖对于谷子产量的提高具有重要意义，但是目前谷子分蘖遗传分子机制还不清楚。利用简化基因组测序技术（RAD-seq），以2个F₂群体（命名为Cross AJ和Cross HC）为研究对象，分别对2个F₂群体的543个和131个单株及其亲本进行RAD-seq，利用MSTmap和WinQTLCart 2.5软件进行遗传图谱构建和QTL分析，结果共鉴定出8个控制分蘖相关的QTL。其中，利用Cross AJ共鉴定了6个QTL，分别为qAJTN1、qAJTN5、qAJTN7-1、qAJTN7-2、qAJTN7-3和qAJTN9，可解释表型变异0.7%~9.8%；利用Cross HC群体共鉴定了2个QTL，分别为qHCTN5和qHCTN7，可解释表型变异1.4%~8.3%。在这8个QTL中，qAJTN1、qAJTN5、qAJTN7-2、qAJTN9和qHCTN5为该研究新鉴定的位点，其余3个QTL（qAJTN7-1、qAJTN7-3和qHCTN7）与前人研究结果相一致。另外，通过将亲本的测序结果与参考基因组进行比对，搜索插入缺失位点InDel（Insertion-deletion），新开发了谷子分蘖相关QTL（qAJTN7-3和qHCTN7）紧密相关的InDel标记。结果为谷子分蘖机制的研究奠定一定基础，所开发的分子标记对于分蘖型谷子的分子标记辅助育种具有重要意义。

关键词: 谷子, 分蘖, QTL定位, 连锁标记

Abstract: Tillering is an important trait related to yield in many crops,and is the same for yield improvement of foxtail millet. However, the genetic mechanism of tillering in foxtail millet (Setaria italica) remains largely unknown. To accelerate mapping of relevant QTLs or genes, a restriction site-associated DNA sequencing (RAD-seq) approach, MSTmap and WinQTLCart 2.5 were employed to identify QTLs responsible for tillering in two F₂ populations called Cross AJ (543 F₂ individuals) and Cross HC (131 F₂ individuals). A total of six QTLs including qAJTN1, qAJTN5, qAJTN7-1,qAJTN7-2, qAJTN7-3 and qAJTN9, were identified in Cross AJ, explaining 0.7%-9.8% of the phenotypic variance, and two QTLs including qHCTN5 and qHCTN7 were identified in Cross HC, explaining 1.4%-8.3% of the phenotypic variance. Of which qAJTN1, qAJTN5, qAJTN7-2, qAJTN9 and qHCTN5 were newly identified QTLs, and qAJTN7-1, qAJTN7-3 and qHCTN7 were consistent with previous reports. Meanwhile, we screened the insertions and deletions in the QTL regions compared with the reference genome, insertion-deletion (InDel) markers linked with tillering were developed. These results of this study will facilitate the revelation of the genetic mechanism of tillering and molecular marker-assisted breeding of foxtail millet.

Key words: Foxtail millet, Tillering, QTL mapping, Linked marker

中图分类号:

S515

杜晓芬, 王军, 李云飞, 王智兰, 袁国保, 杜国华, 韩芳, 彭建祥, 张文娜, 蔡伟, 袁峰, 崔巨多, 郭二虎, 邹洪锋, 张林义, 彭书忠. 谷子分蘖相关QTL定位及紧密连锁标记开发[J]. 华北农学报, 2018, 33(6): 33-41. doi: 10.7668/hbnxb.2018.06.005.

DU Xiaofen, WANG Jun, LI Yunfei, WANG Zhilan, YUAN Guobao, DU Guohua, HAN Fang, PENG Jianxiang, ZHANG Wenna, CAI Wei, YUAN Feng, CUI Juduo, GUO Erhu, ZOU Hongfeng, ZHANG Linyi, PENG Shuzhong. Mapping of Quantitative Trait Locus and Developing of Closely Linked Markers for Tillering in Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(6): 33-41. doi: 10.7668/hbnxb.2018.06.005.

http://www.hbnxb.net/CN/Y2018/V33/I6/33

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