摘要： NaCl筛选法是培养无标记耐盐转基因水稻的有效方法。以中华11、秀水11、春江06这3个粳稻品种成熟胚愈伤组织为材料,以SKC1为目标基因,研究NaCl筛选条件下,热激与液体共培养对农杆菌介导耐盐基因转化水稻效率的影响。结果显示,热激处理和液体共培养都能提高耐盐基因对水稻的转化率,其中,热激处理最高转化提高率达到46.2%,液体共培养后最高转化提高率达到60.7%。

关键词: 水稻, NaCl筛选, 热激, 液体共培养, 转化效率

Abstract: It is an effective method to use NaCl selecting to generate marker-free salt-tolerant transgenic rice. We used the three japonica rice cultivar(ZhongHua11, Xiushui11, Chunjiang06) mature embryos callus as materials, SKC1 as the target gene to study that on the conditions of screening NaCl, heat shock treatment and liquid coculture of Agrobacterium-mediated gene transfer salt-tolerant rice efficiency. The results showed that heat shock and liquid co-culture of rice could enhance the efficiency of salt tolerance gene transfer. Among them, the heat shock treatment increased the maximum efficiency of gene transfer was 46. 2%, while the liquid co-culture increased the highest efficiency of gene transfer was 60. 7%.

Key words: Rice, NaCl screening, Heat shock, Liquid co-culture, The efficiency of gene transfer

中图分类号: 

• Q943

• S511.03