摘要： 以农杆菌介导法将淀粉合成关键酶AGPase（腺嘌呤二磷酸葡萄糖焦磷酸化酶）基因导入云南地方优质水稻合系35，38，39，1，2中，获得了大量的可育转基因植株。PCR扩增检测，外源基因已稳定整合进水稻基因组中。大量的农艺性状及淀粉含量研究表明，转基因水稻种子的千粒重比对照明显提高，但总淀粉含量没有显著变化。

关键词: AGPase基因, 根癌农杆菌, 聚合酶链式反应, 水稻淀粉含量, 千粒重

Abstract: AGPase gene, the critical starch sy nthesis enzyme gene,was transformed into hig h-class rice No.35, 38, 39, 41, 42 by Agrobacterium-mediated transformation and fertile transgenic rice plants were obtained.The results of PCR indicated that the foreign gene had been integ rated into the rice genome.The research showed that 1000-grain weight of transgenic plants was obviously improved, but the starch content of the rice was little changed.

Key words: AGPase gene, Ag robacterium tumefaciens, Polymerase chain reaction (PCR), The starch content of rice, 1000-g rain weight

中图分类号: 

• S511.035.3