摘要： 为了探讨西门塔尔牛乳铁蛋白基因第11外显子多态性,及其与乳房炎和部分乳性状的关系,利用PCR-SS-CP和DNA测序技术,对118头健康牛和38头感染乳房炎牛个体,进行了第11外显子的基因多态性分析。结果发现,健康牛和感染乳房炎牛个体的第11外显子基因型和基因频率分布差异均极显著(P<0.01),感染牛中AA基因型和A基因频率均极显著高于健康牛(P<0.01)。而且,AA基因型个体SCS显著高于AB和BB型个体(P<0.05),AA型个体的乳蛋白极显著高于AB型(P<0.01)、显著高于BB型(P<0.05),而BB型显著高于AB型(P<0.05)。这表明乳铁蛋白基因第11外显子的A基因,可能是一个西门塔尔牛乳房炎的易感基因。
关键词:
牛乳铁蛋白,
体细胞,
乳房炎,
聚合酶链式反应-单链构象多态
Abstract: To explore the association of polymorphisms of bovine lactoferrin(bLF)gene with the susceptibility to?mastitis and part milk trait，PCR-SSCP and DNA sequencing techniques were used to detect the polymorphisms of?exon11 of 38 case cattle and 118 control cattle. The results analysis showed that，the extremely significant differences in genotypic frequencies and allelic frequencies of exon11 were present in case group and control group(P ＜?0. 01)，AA genotypic frequencies and A allelic frequencies of case group were extremely significant higher than the?control group(P ＜0. 01). Moreover，the SCS of AA genotype individuals were significant higher than AB and BB?genotype(P ＜0. 05)，the milk protein of AA genotype individuals were extremely significant higher than AB genotype(P ＜0. 01)，and significant higher than BB genotype(P ＜0. 05)，the BB genotype individuals were significant?higher than AB genotype(P ＜0. 05). These findings indicated a prospect，the A allelic of bLF-exon11 may have relevant to the susceptibility of mastitis in Simmental cattle.
Key words:
Bovine lactoferrin,
Somatic cell count,
Mastitis,
PCR-SSCP
中图分类号:
王国富, 吴慧光, 孙国权, 李俊雅, 高雪, 王景山, 王玉泉, 高树新. 西门塔尔牛bLF-exon11基因多态性及其与乳房炎易感性和部分乳性状的相关分析[J]. 华北农学报, 2012, 27(3): 218-222. doi: 10.3969/j.issn.1000-7091.2012.03.042.
WANG Guo-fu, WU Hui-guang, SUN Guo-quan, LI Jun-ya, GAO Xue, WANG Jing-shan, WANG Yu-quan, GAO Shu-xin. Association Analysis of Polymorphism in the Bovine Lactoferrin Gene Exon11 with the Susceptibility to Mastitis and Part Milk Trait in Simmental Cattles[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 218-222. doi: 10.3969/j.issn.1000-7091.2012.03.042.