华北农学报

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IL-8受体和乳铁蛋白基因多态性与奶牛隐性乳房炎关系的初步研究

何高明, 李大全, 孙庆华   

  1. 石河子大学动物科技学院, 新疆石河子 832003
  • 收稿日期:2009-01-18 出版日期:2009-12-31
  • 作者简介:何高明(1967 -), 男, 重庆江津人, 副教授, 主要从事奶牛营养代谢病方面的研究。
  • 基金资助:
    石河子大学科学研究发展项目(2006)

Preliminary Study of Relationship between the IL-8 Receptor and Lactoferrin Gene Polymorphisms and Subclinical Mastitics -dairy Cows

HEGao-ming, LIDa-quan, SUNQing-hua   

  1. College of Animal Science and Technology of Shihezi University, Shihezi 832003, China
  • Received:2009-01-18 Published:2009-12-31

摘要: 采用PCR-SSCP技术分别对80头健康和隐性乳房炎奶牛的IL-8受体基因、乳铁蛋白基因进行了多态性检测,并与相对应的奶牛乳汁中的体细胞数进行了相关分析,结果表明,两者都与奶牛隐性乳房炎存在关联;在IL-8受体CXCR1基因PCR-SSCP分析中,SSCP5、SSCP7在健康奶牛个体中出现程度较高,说明个体对隐性乳房炎表现较好的抗性;在乳铁蛋白基因5′调控区的不同基因型中,SSCP1对奶牛隐性乳房炎有很好的抗性。

关键词: 隐性乳房炎, IL-8受体基因, 乳铁蛋白基因, PCR-SSCP

Abstract: The IL-8R gene and Lactoferrin gene polymorphisms of 80 healthy and subclinical mastitics -dairy cowswere detected respectively by PCR -SSCP technique,the somatic cells in the milk were counted.The results showed thatthe 2 genes polymorphisms have a certain relationship with the subclinical mastitics,genetypes SSCP -5 and genetypesSSCP -7 of IL -8R CXCR1 with high frequency in the healthy individual dairy cows indicated that they performed arather good resistance to subclinical -mastitics, so was genetypes SSCP -1 in lactoferrin gene 5'regulatory region.

Key words: Subclinicalmastitics, IL-8Rgene, Lactoferringene, PCR-SSCP

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引用本文

HEGao-ming, LIDa-quan, SUNQing-hua. Preliminary Study of Relationship between the IL-8 Receptor and Lactoferrin Gene Polymorphisms and Subclinical Mastitics -dairy Cows[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.058.

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               http://www.hbnxb.net/CN/Y2009/V24/IS1/251