摘要： 采用PCR-SSCP技术对5个牦牛品种共398头牦牛脂蛋白脂肪酶基因(LPL)外显子7进行了多态性研究,分析了该基因与牦牛生长性状的相关性.结果表明,牦牛LPL基因外显子7存在2个等位基因3种基因型.各群体在该基因座上呈Hardy-Weinberg平衡状态.三种基因型与牦牛部分生长性状的最小二乘法分析表明,该位点多态与牦牛的体重、体高、胸围存在显著相关(P<0.05),BB型个体体重、体高、胸围显著高于AA和AB型个体.初步推断牦牛LPL基因第7外显子可作为牦牛标记辅助选择的遗传标记之一.
关键词:
牦牛,
LPL基因,
多态性,
性状标记,
PCR-SSCP
Abstract: The PCR-SSCP polymorphism of Lipoprotein lipase(LPL) gene sequence of part extron 7 and its correlation with growth traits were studied in 398 individuals from five breeds yak. The results showed that there were two alleles and three genotypes in the locus of LPL gene extron 7. They were at Hardy-Weinberg equilibrium state in this locus. The least square analysis showed that the different genotype of LPL extron 7 had a significant effect on partial traits of growth including body weight,heart girth,body height(P 0. 05) ,and the genotype did not affected on body slanting length significantly(P 0. 05) . Therefore,these results imply that the mutation at the extron 7 of yak LPL gene could be used as the molecular genetic marker for yak breeding.
Key words:
Yak,
LPL gene,
Polymorphism,
Genetic marker,
PCR-SSCP
中图分类号:
梁春年, 邢成峰, 阎萍, 丁学智, 裴杰, 郭宪, 包鹏甲, 褚敏, 朱新书. 牦牛LPL基因外显子7多态性与生长性状相关性的研究[J]. 华北农学报, 2010, 25(5): 16-19. doi: 10.7668/hbnxb.2010.05.003.
LIANG Chunnian, XING Chengfeng, YAN Ping, DING Xuezhi, PEI Jie, GUO Xian, BAO Pengjia, CHUMin, ZHU Xinshu. Study on Association of Single Nucleotide Polymorphism at Extron 7 of LPL Gene with Growth Traits in Yak[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 16-19. doi: 10.7668/hbnxb.2010.05.003.