苹果PGIP基因植物表达载体的构建及转化番茄的研究

doi:10.7668/hbnxb.2010.05.009

摘要/Abstract

摘要： 为了验证苹果多聚半乳糖醛酸酶抑制蛋白(PGIP)基因表达产物对植物病原真菌的作用,获得转基因植株,培育抗病新品种,本研究用限制性内切酶SalⅠ、BamHⅠ将已克隆的苹果PGIP基因从克隆载体pMD-18T上切下,定向插入到植物表达载体pWR306的ED3s启动子和TNOS终止子中间,成功构建了苹果PGIP基因植物表达载体pWR306-PGIP及工程农杆菌,以番茄中蔬四号叶片及茎段为受体,通过农杆菌介导法转化,获得了8株PCR检测阳性的番茄植株.

关键词: 多聚半乳糖醛酸酶抑制蛋白基因, 植物表达载体, 根癌农杆菌, 遗传转化, 番茄

Abstract: Aimed to study the function of polygalacturonase-inhibiting protein gene in plant,breed disease resistant cultivar,obtain the transgenic plant,a plant expression vector pWR306-PGIP was constructed by inserting the PGIP gene coding fragment from cloning vector pMD-18T by SalⅠand BamHⅠsites into pWR306 between of ED35s promotor and NOS terminator. The identification results showed that the plant expression vector pWR306-PGIP and Agrobacterium tumefaciems were constructed successfully. Subsequently PGIP gene was transformed into Zhongshu No. 4 tomato leaves and shoot fragments mediated by Agrobacterium tumerfaciens. Eight transgenic resistant plants were proved by PCR preliminarily that the PGIP gene had integrated into tomato genome

Key words: PGIP gene, Plant expression vector, Agrobacterium tumerfaciens, Genetic transformation, Tomato

中图分类号:

石玉, 张军科, 张毅, 洪晓娟, 马锋旺. 苹果PGIP基因植物表达载体的构建及转化番茄的研究[J]. 华北农学报, 2010, 25(5): 42-46. doi: 10.7668/hbnxb.2010.05.009.

SHI Yu, ZHANG Junke, ZHANG Yi, HONG Xiaojuan, MA Fengwang. Plant Expression Vector Construction of Apple PGIP Gene and Its Transformation into Tomato[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 42-46. doi: 10.7668/hbnxb.2010.05.009.

http://www.hbnxb.net/CN/Y2010/V25/I5/42

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