摘要： 2011-2012年,河南省大面积暴发商品蛋鸡群肿瘤病,临床剖检疑似马立克氏病(Marek’s disease,MD),为进一步确诊病因,根据马立克氏病病毒(Marek’s disease virus,MDV)的meq、gB和pp38基因分别设计了3对特异性引物,利用PCR技术对送检的疑似病例鸡的肝脏和脾脏样品分别进行了检测和分析。结果显示,各地区随机抽取的5只病例鸡肝脏、脾脏样品中均扩增出3种不同大小的MDV特异性病毒基因PCR产物,这表明MDV在河南鸡群中广泛流行,并导致了商品蛋鸡群MD的暴发。研究结果填补了河南省MD流行病学空白,为今后MD的临床快速诊断及有效防控提供了重要参考依据。
关键词:
马立克氏病,
流行病学,
马立克氏病病毒,
聚合酶链式反应
Abstract: In 2011-2012， the tumorigenic and immunosuppresive disease were broken out in the commercial layer chickens in large areas of Henan province． The clinical symptoms and macroscopic pathological inspections suggested that it might be a case of Marek's disease ( MD). In order to identity the cause of the disease， three pairs of primers specific to the MDV meq，gB and pp38genes were designed according to the genomic sequence of MDV strain GA． Meanwhile，polymerase chain reaction ( PCR) was carried out on liver and spleen samples of those suspected cases． The results showed that three different sizes of PCR products of MDV-specific genes were successfully amplified from the randomly tested five cases collected in each region． It indicated that MDV was widely spread in Henan Provice and led to the outbreak of MD in the commercial layer chickens． The study provided useful information for the rapid diagnosis and efficient control of MD in the future．
Key words:
Marek's disease,
Epidemiology,
MDV,
PCR
中图分类号:
禹乐乐, 滕蔓, 罗俊, 王新卫, 宿靖伟, 柴书军, 王爱萍, 张改平. 河南商品蛋鸡群中鸡马立克氏病的流行病学研究[J]. 华北农学报, 2012, 27(6): 163-166. doi: 10.3969/j.issn.1000-7091.2012.06.032.
YU Le-le, TENG Man1, LUO Jun, WANG Xin-wei, SU Jing-wei, CHAI Shu-jun, WANG Ai-ping, ZHANG Gai-ping. Epidemiology of Marek's Disease in Commercial Layer Chickens in Henan Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 163-166. doi: 10.3969/j.issn.1000-7091.2012.06.032.