摘要： 根据鸡传染性贫血病毒(CIAV)基因组序列,设计一对引物扩增基因组上nt58～nt1042之间684 bp DNA片段.在CIAV感染MDCC-MSB1细胞系中提取核酸为模板可扩增出相应DNA片段,且以感染细胞核酸为模板反应敏感性可达1 fg,而以其他病原核酸为模板扩增结果均为阴性.应用该PCR方法对实验感染鸡肝脏、脾脏、胸腺、肾脏、法氏囊、骨髓、白细胞、泄殖腔棉拭子等不同样品进行检测,均可扩增出相应DNA片段.证明我们建立的PCR方法敏感性特异性强,可用于临床感染不同组织CIAV的检测.

关键词: 鸡, 传染性贫血病, 病毒, 聚合酶链式反应

Abstract: A polymerase chain reaction (PCR) was constructed to detect chicken infectious anemia virus (CIAV). The assay used a pair of primers to amplify a 684 bp fragment of CIAV genome which located in the position 358 to 1 042. The amplified fragment was observed to detect infected cell culture and the sensitivity of the assay can be up to 1 fg. The result of amplification with NDV,MDV,IBDV, IBV,EDSV and E. Coli were negative. It showed that the assay was specific and highly sensitive. The positive fragments sere also be achieved in infected chicken tissues such as liver, spleen, thymus, bursa, kidney, white blood cell and rectal sub.

Key words: Chicken, Chicken infectious anemia, Virus, PCR

中图分类号: 

• S858.1