华北农学报 ›› 2001, Vol. 16 ›› Issue (1): 73-77. doi: 10.3321/j.issn:1000-7091.2001.01.014

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应用浸种法导入外源DNA转化烤烟遗传性状变异初探(Ⅱ)

朱生伟1, 徐仲2, 史芝文2, 张寒霜3   

  1. 1. 中国科学院植物所, 北京 100093;
    2. 东北农业大学生物工程学院, 黑龙江哈尔滨 150030;
    3. 河北省农林科学院棉花所, 河北石家庄 050031
  • 收稿日期:1999-07-04 出版日期:2001-03-28
  • 作者简介:朱生伟(1971- ),男,博士,助研,主要从事植物基因工程方面研究工作。

A Preliminary Study by Using Seed Soaking Method of Introduce Exogenous DNA to Induce the Genetic Characters in Flue-cured Tobacco()

ZHU Shengwei1, XU Zhong2, ZHANG Hanshuang2, SHI Zhiwen3   

  1. 1. Institute of Botany, Academia Sinica, Beijing 10009
    3. , China;
    2. Northeast Agricultural University, Harbin 150030, China;
    3. Cotton Institute, Heibei Academy of Agricultural and Forestry Scienc
  • Received:1999-07-04 Published:2001-03-28

摘要: 对应用浸种处理获得的变异后代,进行了叶绿素、糖、蛋白质、维生素C及株高、叶面积、生育期等的测定。试验表明:该方法对株高、叶面积、生育期等性状的影响较为显著,对叶绿素、糖、蛋白质及维生素C等的改变不明显。

关键词: 烤烟, 浸种法, 外源DNA导入, 变异

Abstract: In this experiment,the variant materials obtained by seed soaking method were tested for biochemical indices such as chlorophyll,soluble sugar,reducing sugar,protein and Vitamin C contents,as well as plant height,plant type,leaf area and growth period.The results showed that plant height,plant type,leaf area,growth period and disease resistance were significantly affected. However,the contents of chlorophyll,soluble sugar ,reducing sugar,protein and Vitamin C were not.The method has the advantages of high ratio of variation(21.2%),easy to operate,and the separations stablize quickly.

Key words: Flue-cured tobacco, Seed soaking method, Exogenous DNA Introduction, Variance

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引用本文

朱生伟, 徐仲, 史芝文, 张寒霜. 应用浸种法导入外源DNA转化烤烟遗传性状变异初探(Ⅱ)[J]. 华北农学报, 2001, 16(1): 73-77. doi: 10.3321/j.issn:1000-7091.2001.01.014.

ZHU Shengwei, XU Zhong, ZHANG Hanshuang, SHI Zhiwen. A Preliminary Study by Using Seed Soaking Method of Introduce Exogenous DNA to Induce the Genetic Characters in Flue-cured Tobacco()[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(1): 73-77. doi: 10.3321/j.issn:1000-7091.2001.01.014.

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