摘要： 将类甜蛋白基因（TLP）及其紧密连锁的抗除草剂bar基因以农杆菌介导法导入脱毒微型种薯“津引薯8号”，经除草剂筛选后的转基因单株无性繁殖用于分子检测，标记基因bar基因的PCR反应和含TLP基因特异探针的Southern检测结果，转基因植株分别显示出0.54kb和3.0kb的特异性条带，证明TLP基因已成功地整合到转基因马铃薯基因组中。利用TLP基因特异性抗血清对转基因植株进行Western反应检测，结果表明，转基因植株中TLP基因表达量显著高于对照组，经晚疫病菌游离孢子接种离体抗性分析，转基因株系表现出对晚疫病的明显抑制作用和症状发生的延迟作用。

关键词: 马铃薯晚疫病, 类甜蛋白基因, 表达, 抗病性

Abstract: Thaumat in??like protein ( TLP) gene and bar gene which is closely combined with TLP gene were transferred into virus free micropotato variety Jing Yinshu 8 by agrobacterium??mediated transformat ion. Transgenic plants selected by bialaphos were amplified for detection. There sults of PCR of maker bar gene and Southern blot of specific probe with TLP gene showed that transgenic plants displayed 0.54 kb and 3.0 kb bands, respectively, indicating TLP gene has been successfully integ rated into the chromosome of transgenic potatoes. The western reaction of trans genic plants using TLP antiserum showed the expression of TLP geneint ransg enic plans was much higher than the control. Through the vitro assay of leaves inoculated by alive spores of phytophthora, leaves of transgenic potato plant s exhibited significant inhibition on late blight disease and delayed development of disease symptoms.

Key words: Potato late-blight, Thaumat in-like protein(TLP) gene, Expression, Resistance

中图分类号: 

• S532.035.3