赤霉素反应敏感型和不敏感型谷子矮秆突变体的鉴定

doi:10.3321/j.issn:1000-7091.1998.01.009

华北农学报 ›› 1998, Vol. 13 ›› Issue (1): 46-52. doi: 10.3321/j.issn:1000-7091.1998.01.009

所属专题： 杂粮作物；

赤霉素反应敏感型和不敏感型谷子矮秆突变体的鉴定

陈金桂¹, 张玉宗², 周燮¹

1. 南京农业大学农学系农业部作物生长调控重点开放实验室;
2. 河北省农林科学院谷子研究所

收稿日期:1996-07-02 出版日期:1998-03-28
基金资助:
河北省自然科学基金

Characterization of Gibberellin-Responding and Non-Responding Dwarf Mutants in Foxtail Millet

Chen Jingui¹, Zhang Yuzong², Zhou Xie¹

1. Key Laboratory of Crop Growth Regulation,Ministry of Agriculture,Department of Agronomy,Nanjing Agricultural University,Nanjing　210095;
2. Institute of Millet,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang　050031

Received:1996-07-02 Published:1998-03-28

摘要/Abstract

摘要： 采用赤霉素点滴法（苗期）、浸渍法（芽期）以及喷洒法（拔节期）对3种谷子矮秆突变体的赤霉素反应敏感性进行了研究。结果表明矮宁黄和623C属赤霉素反应敏感型，它们苗期的叶片和叶鞘及拔节期的节间均可随外加赤霉素而显着伸长，达到正常株（苗）高；CH84113则属于赤霉素反应不敏感型，多种赤霉素（GA1、GA3、GA4、GA7、GA9）处理均不能使其株（苗）高恢复正常。用ELISA对3种矮秆突变体和正常品种伸长敏感期（拔节期）幼茎的GA1+3含量进行了检测，发现矮宁黄、623C和CH84113的GA1+3含量均存在着显着的亏缺。

关键词: 谷子, 矮化, 突变体, 赤霉素, 敏感性

Abstract: Seedlings of foxtail millet(Setaria italica Beauv.)dwarf mutants,Aininghuang( ANH),623C and CH84113 were tr eated with gibberellins (GA₁,GA₃,GA₄,GA₇ and GA₉)by micro-drop method and by pre-soaking in GA₃ solution.Further,plants were sprayed with GA₃ solution at jointing stage.It was found that ANH and 623C were GA- respondingdwarf mutants,whose leaf blade,leaf sheath and elongating internodes lengh increased significantly after GA application,whereas CH84113 was anon- GA-responding dwarf mutant.Using on enzyme-linked immunosorbent assay(ELISA),it was found that the endogenous GA₁₊₃ contents inculms of all three dwarf mutants decreased by 50% or so in comparing with the normal cultivars,Jigu11(JG11),Zhaogu1 (ZG1) and Qingfenggu(QFG).

Key words: Foxtail millet(Setaria italica Beauv), Dwarfism, Mutant, Gibberellins, Sensitivity

中图分类号:

S511

陈金桂, 张玉宗, 周燮. 赤霉素反应敏感型和不敏感型谷子矮秆突变体的鉴定[J]. 华北农学报, 1998, 13(1): 46-52. doi: 10.3321/j.issn:1000-7091.1998.01.009.

Chen Jingui, Zhang Yuzong, Zhou Xie. Characterization of Gibberellin-Responding and Non-Responding Dwarf Mutants in Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(1): 46-52. doi: 10.3321/j.issn:1000-7091.1998.01.009.

http://www.hbnxb.net/CN/Y1998/V13/I1/46

参考文献

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[8] Fujioka S,Yamane H,Spray CR,etal.The dominant non-gibber ellin-responding dwarf mutant(D8)of maize accumulat es native gibberellins.Proc NatlAcad Sci USA,1988,85:9031～9035
[9] Phinney BO,Spray CR.Chemical genetic and the gibberellin pathway in Zeamays L.In:Wareing PF,ed.Plant Growth Substances 1982,New York:Academic Press,1982,101～110
[10] Reid JB,Davies PJ.The genetic and physiology of gibberellin sensitivity mutants in peas.In:KarssenCM,vanLoon LC,Vreugdenhil D,eds.Progress in Plant Growth Regulation.Netherland:Kluwer Academic Publishers,1992,214～225
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[13] Zheng ZF,Zhou X. Amonoclonal antibody recognizing non-derivative 13-hydroxcy gibberellin A3 andtheir glucosides. Act aBotania Sinica,1995,37(10):761～769
[14] Yamaguchi I,Nakazawa H,Nakagawa R,etal.Identification and semi-quantification of gibber ellins from the pollen and anthers of Zeamays by immuno assay and GC/MS.Plant Cell Physiol,1990,31(8):1063～1069
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赤霉素反应敏感型和不敏感型谷子矮秆突变体的鉴定

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赤霉素反应敏感型和不敏感型谷子矮秆突变体的鉴定

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