华北农学报 ›› 2020, Vol. 35 ›› Issue (6): 36-41. doi: 10.7668/hbnxb.20191175

所属专题: 杂粮作物 抗旱节水

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

谷子萌芽期抗旱相关QTL定位研究

代小冬, 朱灿灿, 王春义, 秦娜, 宋迎辉, 代书桃, 李君霞   

  1. 河南省农业科学院 粮食作物研究所, 河南 郑州 450002
  • 收稿日期:2020-08-13 出版日期:2020-12-28
  • 通讯作者: 李君霞(1972-),女,河南禹州人,副研究员,硕士,主要从事谷子新品种选育及栽培研究。
  • 作者简介:代小冬(1981-),男,河南正阳人,副研究员,博士,主要从事谷子新品种选育及栽培研究。
  • 基金资助:
    国家自然科学基金项目(U1504314);国家现代农业产业技术体系专项资金(nycytx-CARS-06)

Mapping of Drought Resistance QTLs at Germination Stage of Foxtail Millet

DAI Xiaodong, ZHU Cancan, WANG Chunyi, QIN Na, SONG Yinghui, DAI Shutao, LI Junxia   

  1. Cereal Crops Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
  • Received:2020-08-13 Published:2020-12-28

摘要: 为解析谷子抗旱遗传基础和指导抗旱育种,进行谷子萌芽期抗旱基因定位,以山西2010和K359×M4-1为亲本构建的F2群体为研究对象,结合利用2b-RAD测序技术构建的遗传连锁图谱对谷子萌芽期抗旱性QTL进行定位。谷子萌芽期抗旱性为多基因控制的数量性状。经两亲本及F2群体简化基因组测序,构建了包含583个SNP标记的连锁图谱。该图覆盖谷子基因组9条染色体,各染色体标记数平均64.8,标记间平均间隔为0.97 cM。在第5和第6染色体上共检测到3个谷子萌芽期抗旱性相关QTL,分别为qSIDR-5a、qSIDR-6aqSIDR-6b,可解释12.4%~14.3%的表型变异,其中第5染色体上qSIDR-5a的表型贡献率最高,达到14.3%。经过比较发现,这3个QTL与前人的研究结果不在一个区间内,是新的QTL。qSIDR-5a、qSIDR-6aqSIDR-6b可用于后续萌芽期抗旱性调控基因的精细定位及克隆研究,也可用于谷子萌芽期抗旱性的分子调控机理研究与抗旱育种。

关键词: 谷子, 萌芽期, 抗旱性, QTL

Abstract: In order to explore the genetic basis of drought resistance and guide drought resistance breeding, mapping of QTL associated with drought resistance at germination stage of foxtail millet was done. An F2 segregating population was constructed using 100 individuals derived from a cross between Shanxi 2010 and K359×M4-1. A genetic linkage map was constructed based on 2b-RAD sequencing, which was then combined with the phenotypic trait of drought resistance for QTL mapping. The results showed that drought resistance at germination stage of foxtail millet was a complex quantitative trait and controlled by multiple genes. A genetic map containing 583 SNP makers was constructed by 2b-RAD of parents and F2 population. The map covered 9 chromosomes of foxtail millet, with an average number of 64.8 and an average genetic distance of 0.97 cM between markers. A total of 3 QTLs were identified:qSIDR-5a, qSIDR-6a and qSIDR-6b, located in foxtail millet chromosome 5 and 6, respectively, which explained 12.4%-14.3% of phenotypic variation. Among them, phenotypic contribution rate of qSIDR-5a was the highest, which explained 14.3% of phenotypic variation. These QTLs were not included in the same chromosome interval as the identified drought resistance related QTLs of foxtail millet, and thus were described as new candidate gene loci associated with germination stage drought resistance.These QTLs can be used for fine mapping and gene cloning, as well as molecular regulation mechanism of drought resistance of foxtail millet.

Key words: Foxtail millet, Germination stage, Drought resistance, QTL

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引用本文

代小冬, 朱灿灿, 王春义, 秦娜, 宋迎辉, 代书桃, 李君霞. 谷子萌芽期抗旱相关QTL定位研究[J]. 华北农学报, 2020, 35(6): 36-41. doi: 10.7668/hbnxb.20191175.

DAI Xiaodong, ZHU Cancan, WANG Chunyi, QIN Na, SONG Yinghui, DAI Shutao, LI Junxia. Mapping of Drought Resistance QTLs at Germination Stage of Foxtail Millet[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 36-41. doi: 10.7668/hbnxb.20191175.

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