摘要： 以水稻野败型与BT型细胞质雄性不育系及其保持系（珍汕97A,B,花76─49A,B）为材料，用酶联免疫检测法（EL1SA）测定了幼德与倒二叶中内源GA₁₊₄与IAA含量。结果表明，珍汕97A与花76-49A倒二叶中的GA₁₊₄含量低于各自保持系的，珍汕97A幼穗GA₁₊₄含量低于其保持系的，而花76-49A的幼穗GA₁₊₄含量近似于其保持系的。与此不同，珍汕97A与花76─49A的幼穗与倒二叶中IAA含量则均显着低于各自保持系的，其中珍汕97A的IAA含量显着降低的时期早于花76-49A的，从而提示GA₁₊₄与IAA亏缺是导致水稻细胞质雄性不育的一种生理原因。

关键词: 水稻, 细胞质雄性不育, 赤霉素, 吲跺乙酸

Abstract: GA₁₊₄ and IAA contents of Wild-type and BT-type cytoplasmic male sterile lines of rice and their restores(Zhen 97A.B;Hua 76-49A,B)were detected with indirect enzyme-linked-immunosorbent-assay(ELISA).It was found that GA₁₊₄ contents in the penultimate lamina of Zhen 97 A and Hua 76-49A were lower than that of their restore lines since the beginning of spikelet-differentiation stage.From reduction-division stage,GA₁₊₄ content in young panicle of Zhen 97A became lower than that of its restore line,while GA₁₊₄ content in young pancile of Hua 76-49A was still similar to that of its restore line.It was also found that IAA contents in pancile and penultimate lamina of Zhen 97A were obviously decreased compared with that of their restore lines since spikelet-differentiation stage and the beginning of IAA deficiency in Zhen 97A was much earlier than that in Hua 76-49A.It was proposed that GA₁₊₄ and IAA deficiency would be the main physiological reason in rice cytoplasmic male sterility.

Key words: Rice, Cytoplasmic male sterility, GA, IAA

中图分类号: 

• S511.01