摘要： 通过近三年试验，研制出鸡传染性支气管炎毒株IB H₁₂₀细胞弱毒苗，经室内外近40万羽雏鸡免疫试验表明：该苗以每羽0.01ml滴鼻、点眼或口服免疫4~12日龄雏鸡，7d后产生免疫力；免疫后17d、32d、60d抗标准强毒攻击的免疫保护率分别为88.7%、87.5%、87.5%，免疫期可达二个月；其最小免疫量为0.001ml/羽；以10个免疫剂量接种雏鸡无任何不良反应，安全性能良好；-20℃保存半年、4℃保存一个月效力不变。

关键词: 鸡传染性支气管炎, 鸡全胚细胞培养, 疫苗

Abstract: The attenuated cell-culture vaccine of avian infectious bronchitis H₁₂₀ virus wassuccessfully prepared. Immune trials carried out in nearly 400 thousand chicks with the vaccine in and out of the laboratory indicated that the immunity was obtained in 4 to 12 day-old chicks 7 days after vaccination at a dose of 0.01 ml each by the way of nasaldrop,eyedrop or drinking-water,and that the protection rates of 88.7% ,87.5% and 87.5% were respectively provided for the chicks,which were inoculated for 17,32 and 60 days,while they were challenged with the standard virulent IBV. The immune protection period of the vaccine for chicken was two months. No harmful effect was observed in the 4 to 12 days-old chicks within 15 days after inoculation with 10 immune doses of the vaccine. The minimum immune dose of the vaccine is 0.001 ml per chick. The vaccine is stored at-20℃ for half a year or at 4℃ for one month and the effect is not weakened.

Key words: Avian infectious bronchitis, H₁₂₀ virus, Vaccine, Whole-chicken-embryo cell culture